Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BZG32_RS12640 Genome accession   NZ_CP019512
Coordinates   2439635..2441446 (-) Length   603 a.a.
NCBI ID   WP_002356480.1    Uniprot ID   -
Organism   Enterococcus faecalis strain CLB21560     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2434635..2446446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZG32_RS12615 (BZG32_12620) - 2435460..2436257 (-) 798 WP_002356486.1 NAD kinase -
  BZG32_RS12620 (BZG32_12625) - 2436270..2436944 (-) 675 WP_002356484.1 GTP pyrophosphokinase family protein -
  BZG32_RS12625 (BZG32_12630) - 2437141..2437719 (+) 579 WP_002356483.1 CYTH domain-containing protein -
  BZG32_RS12630 (BZG32_12635) - 2437783..2438442 (+) 660 WP_002359562.1 ClpXP adapter SpxH family protein -
  BZG32_RS12635 (BZG32_12640) - 2438725..2439444 (-) 720 WP_002359561.1 helix-turn-helix transcriptional regulator -
  BZG32_RS12640 (BZG32_12645) pepF 2439635..2441446 (-) 1812 WP_002356480.1 oligoendopeptidase F Regulator
  BZG32_RS12645 (BZG32_12650) - 2441494..2442678 (-) 1185 WP_002359560.1 competence protein CoiA -
  BZG32_RS12650 (BZG32_12655) - 2442767..2443429 (-) 663 WP_002356478.1 adaptor protein MecA -
  BZG32_RS12655 (BZG32_12660) spxA 2443772..2444170 (-) 399 WP_002356477.1 transcriptional regulator SpxA -
  BZG32_RS12660 (BZG32_12665) trpS 2444530..2445540 (+) 1011 WP_002359559.1 tryptophan--tRNA ligase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68835.12 Da        Isoelectric Point: 4.7145

>NTDB_id=216141 BZG32_RS12640 WP_002356480.1 2439635..2441446(-) (pepF) [Enterococcus faecalis strain CLB21560]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLTEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=216141 BZG32_RS12640 WP_002356480.1 2439635..2441446(-) (pepF) [Enterococcus faecalis strain CLB21560]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTAACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGACAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGAACCTTAGGGA
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CCAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGGTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGTGGCGCATATTCTTCAGGAGCTTATGACACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CTGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAACTACCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.637

98.342

0.537

Multiple sequence alignment