Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   BZG32_RS09760 Genome accession   NZ_CP019512
Coordinates   1871524..1871799 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain CLB21560     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1866524..1876799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZG32_RS09715 (BZG32_09720) - 1866623..1866901 (+) 279 WP_002380425.1 hypothetical protein -
  BZG32_RS09725 (BZG32_09730) - 1867079..1867552 (+) 474 WP_002357065.1 universal stress protein -
  BZG32_RS09730 (BZG32_09735) - 1867664..1868851 (-) 1188 WP_002357064.1 acetate/propionate family kinase -
  BZG32_RS09735 (BZG32_09740) - 1868876..1869883 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  BZG32_RS09740 (BZG32_09745) comGG 1870012..1870365 (-) 354 WP_002360027.1 competence type IV pilus minor pilin ComGG -
  BZG32_RS09745 (BZG32_09750) comGF 1870365..1870799 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  BZG32_RS09750 (BZG32_09755) - 1870789..1871115 (-) 327 WP_002370967.1 type II secretion system protein -
  BZG32_RS09755 (BZG32_09760) comGD 1871081..1871527 (-) 447 WP_002360025.1 competence type IV pilus minor pilin ComGD -
  BZG32_RS09760 (BZG32_09765) comGC/cglC 1871524..1871799 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  BZG32_RS09770 (BZG32_09775) comGB 1871799..1872844 (-) 1046 Protein_1793 competence type IV pilus assembly protein ComGB -
  BZG32_RS09775 (BZG32_09780) comGA 1872801..1873769 (-) 969 WP_002360023.1 competence type IV pilus ATPase ComGA -
  BZG32_RS09780 (BZG32_09785) - 1874010..1875338 (-) 1329 WP_002360022.1 APC family permease -
  BZG32_RS09790 (BZG32_09795) rlmN 1875628..1876701 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=216136 BZG32_RS09760 WP_002356991.1 1871524..1871799(-) (comGC/cglC) [Enterococcus faecalis strain CLB21560]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=216136 BZG32_RS09760 WP_002356991.1 1871524..1871799(-) (comGC/cglC) [Enterococcus faecalis strain CLB21560]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment