Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BXU06_RS01715 Genome accession   NZ_CP019509
Coordinates   378404..379831 (-) Length   475 a.a.
NCBI ID   WP_077296239.1    Uniprot ID   A0A1U9JIY1
Organism   Aquaspirillum sp. LM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 373404..384831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BXU06_RS01680 (BXU06_01685) - 373906..374538 (-) 633 WP_077296227.1 flavin prenyltransferase UbiX -
  BXU06_RS01685 (BXU06_01690) - 374535..375065 (-) 531 WP_077296229.1 Gx transporter family protein -
  BXU06_RS01690 (BXU06_01695) - 375071..375463 (-) 393 WP_253189498.1 NusG domain II-containing protein -
  BXU06_RS01695 (BXU06_01700) rapZ 375460..376290 (-) 831 WP_077296231.1 RNase adapter RapZ -
  BXU06_RS01700 (BXU06_01705) hprK 376271..377227 (-) 957 WP_077296233.1 HPr(Ser) kinase/phosphatase -
  BXU06_RS01705 (BXU06_01710) ptsN 377220..377681 (-) 462 WP_077296235.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BXU06_RS01710 (BXU06_01715) hpf 377879..378211 (-) 333 WP_077296237.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BXU06_RS01715 (BXU06_01720) rpoN/rpoN1 378404..379831 (-) 1428 WP_077296239.1 RNA polymerase factor sigma-54 Machinery gene
  BXU06_RS01720 (BXU06_01725) lptB 379861..380589 (-) 729 WP_077296241.1 LPS export ABC transporter ATP-binding protein -
  BXU06_RS01725 (BXU06_01730) lptA 380586..381128 (-) 543 WP_171982080.1 lipopolysaccharide transport periplasmic protein LptA -
  BXU06_RS01730 (BXU06_01735) lptC 381109..381684 (-) 576 WP_077296243.1 LPS export ABC transporter periplasmic protein LptC -
  BXU06_RS01735 (BXU06_01740) kdsC 381681..382205 (-) 525 WP_077296245.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BXU06_RS01740 (BXU06_01745) - 382226..383209 (-) 984 WP_077296247.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 52656.53 Da        Isoelectric Point: 4.7764

>NTDB_id=216065 BXU06_RS01715 WP_077296239.1 378404..379831(-) (rpoN/rpoN1) [Aquaspirillum sp. LM1]
MKQSLQLKVSQQLTLTPQLQQSIRLLQLSTLDLQQEIENILHDNPMLERDEGQPDPLPNTADTPASSADTPGNDNNDAPE
PSRDDDGPADSLELSDWSSSGSGNHDDDFDPHANMVAEKTLREHLLDQVAEHALQARDRAVVTFLIEELDDDGYLTQSLE
ELAAHLPEELGVDADELQVGLRLLQQFEPAGVGARSLAESLTLQLAILPDSPVRTLAKVLINEHLSLLAHRDFNKLKRQL
HCDDDMLKAVTLLVASLNPRPAAGFGRDNTQYIVADVMVHKGPEGWRAQLNPAAMPKLRVNSLYAHLLQHHREGGSQLTA
QLQEAKWLIRNVQQRFDTILRVSEAIVARQQQFFELGEVGMRPLVLRDIADELGLHESTISRVTTQKFLLCPRGIFELKY
FFGSALETDSGGECSATAIKARLRLLIGNEDPKKPLSDSALADMLGAEGIQVARRTVAKYREAMQIPPVNLRKSI

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=216065 BXU06_RS01715 WP_077296239.1 378404..379831(-) (rpoN/rpoN1) [Aquaspirillum sp. LM1]
ATGAAACAGAGCCTGCAACTTAAAGTCAGCCAGCAGCTGACCCTGACCCCGCAACTGCAACAGTCGATCCGGCTCTTGCA
GCTTTCCACCTTGGACTTGCAGCAGGAAATTGAAAACATCCTGCACGACAACCCGATGCTGGAGCGTGACGAAGGCCAGC
CCGATCCCCTGCCCAATACGGCGGACACCCCGGCCAGCAGCGCCGACACGCCCGGCAACGACAATAACGACGCGCCAGAG
CCCAGCCGCGACGACGACGGCCCAGCCGATTCGCTGGAGCTGTCCGACTGGTCCAGCAGCGGTTCGGGCAACCACGACGA
CGATTTCGACCCGCACGCCAATATGGTGGCGGAAAAAACCCTGCGCGAGCACCTGCTTGACCAGGTGGCCGAACACGCGC
TGCAAGCGCGCGACCGCGCCGTGGTCACCTTCCTGATCGAAGAACTCGACGACGATGGCTATCTCACCCAGTCGCTGGAA
GAACTCGCCGCCCACCTGCCGGAAGAGCTCGGCGTGGACGCCGACGAACTGCAAGTGGGCCTGCGCCTGTTGCAGCAGTT
TGAACCGGCGGGCGTGGGGGCCCGCTCGCTGGCCGAGTCGCTGACCCTGCAACTGGCCATCCTGCCGGACAGTCCGGTGC
GCACGCTGGCCAAGGTGCTGATCAACGAACACCTGTCGCTCTTGGCGCACCGCGATTTCAACAAGCTCAAGCGCCAGCTG
CACTGTGACGACGACATGCTCAAGGCGGTCACCCTGCTGGTGGCCTCGCTCAACCCGCGCCCGGCGGCCGGTTTTGGTCG
CGACAACACCCAGTACATCGTGGCCGACGTGATGGTGCACAAAGGCCCGGAAGGCTGGCGCGCCCAGCTCAACCCGGCGG
CGATGCCCAAGCTGCGGGTGAATTCGCTGTACGCCCATCTGCTGCAACACCACCGCGAAGGCGGCTCGCAGCTGACTGCG
CAGTTGCAGGAAGCCAAATGGCTGATCCGCAATGTGCAGCAGCGCTTTGACACCATCCTGCGCGTATCCGAGGCCATCGT
GGCGCGCCAGCAACAGTTTTTTGAGCTGGGCGAGGTGGGCATGCGCCCGCTGGTGCTGCGCGACATTGCCGACGAACTTG
GCCTGCACGAATCCACCATCTCGCGGGTGACCACGCAAAAATTCCTTTTATGTCCACGGGGTATCTTCGAACTGAAATAT
TTCTTCGGCAGCGCGCTGGAAACCGATTCCGGCGGAGAATGCTCGGCCACTGCCATCAAGGCGCGGCTGCGCCTGCTGAT
TGGCAACGAAGACCCGAAAAAACCGCTTTCCGATTCGGCATTGGCTGATATGCTGGGCGCTGAAGGCATTCAGGTGGCGC
GGCGGACGGTGGCCAAGTACCGCGAAGCCATGCAGATTCCGCCGGTCAACCTGCGCAAGTCGATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(114-304)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9JIY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.2

100

0.571

Multiple sequence alignment