Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMB_RS03985 Genome accession   NC_003112
Coordinates   797115..798245 (+) Length   376 a.a.
NCBI ID   WP_002225431.1    Uniprot ID   A0A112PMI3
Organism   Neisseria meningitidis MC58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792115..803245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS11915 - 792997..793131 (-) 135 WP_257390589.1 hypothetical protein -
  NMB_RS03970 (NMB0765) lepB 793147..794166 (-) 1020 WP_002244066.1 signal peptidase I -
  NMB_RS03975 (NMB0766) lepA 794309..796102 (-) 1794 WP_002225433.1 translation elongation factor 4 -
  NMB_RS03980 (NMB0767) - 796246..796947 (-) 702 WP_002244067.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMB_RS03985 (NMB0768) pilU 797115..798245 (+) 1131 WP_002225431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMB_RS03990 (NMB0769) - 798279..799256 (+) 978 WP_002222732.1 DNA polymerase III subunit delta' -
  NMB_RS03995 (NMB0770) - 799260..799610 (+) 351 WP_002214009.1 PilZ domain-containing protein -
  NMB_RS04000 (NMB0771) - 799615..800394 (+) 780 WP_002225430.1 TatD family hydrolase -
  NMB_RS04005 (NMB0772) - 800420..800635 (+) 216 WP_002225429.1 putative hemolysin -
  NMB_RS04010 (NMB0773) grxD 800736..801047 (+) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMB_RS04015 (NMB0774) upp 801153..801779 (+) 627 WP_002225428.1 uracil phosphoribosyltransferase -
  NMB_RS04020 (NMB0775) - 801802..802122 (+) 321 WP_002222730.1 DUF2322 family protein -
  NMB_RS04025 (NMB0776) - 802373..802792 (+) 420 WP_002222729.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42245.34 Da        Isoelectric Point: 6.2885

>NTDB_id=21594 NMB_RS03985 WP_002225431.1 797115..798245(+) (pilU) [Neisseria meningitidis MC58]
MFTDENMTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRTITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=21594 NMB_RS03985 WP_002225431.1 797115..798245(+) (pilU) [Neisseria meningitidis MC58]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACCAAAACAAAGGTTCCGA
CCTGTTCGTGACAACCCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TACGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCAGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGTAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCTGATGTCATCCTTATCGGCG
AAATCCGTGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A112PMI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Acinetobacter baylyi ADP1

43.333

87.766

0.38
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment