Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BUW96_RS26455 Genome accession   NZ_CP019325
Coordinates   5744751..5746196 (+) Length   481 a.a.
NCBI ID   WP_076816968.1    Uniprot ID   -
Organism   Achromobacter insolitus strain DSM 23807     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5739751..5751196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUW96_RS26440 (BUW96_26440) - 5740470..5741798 (+) 1329 WP_042795749.1 amidase -
  BUW96_RS26445 (BUW96_26445) leuA 5741902..5743614 (-) 1713 WP_042795748.1 2-isopropylmalate synthase -
  BUW96_RS26450 (BUW96_26450) - 5744123..5744752 (-) 630 WP_042795747.1 hypothetical protein -
  BUW96_RS26455 (BUW96_26455) rpoN/rpoN1 5744751..5746196 (+) 1446 WP_076816968.1 RNA polymerase factor sigma-54 Machinery gene
  BUW96_RS26460 (BUW96_26460) - 5746330..5746611 (+) 282 WP_042795745.1 (2Fe-2S)-binding protein -
  BUW96_RS26465 (BUW96_26465) - 5746642..5747499 (-) 858 WP_042795744.1 LysR substrate-binding domain-containing protein -
  BUW96_RS26470 (BUW96_26470) - 5747630..5748544 (+) 915 WP_042795742.1 DMT family transporter -
  BUW96_RS26475 (BUW96_26475) bfr 5748920..5749396 (+) 477 WP_042795741.1 bacterioferritin -
  BUW96_RS26480 (BUW96_26480) - 5749473..5750156 (-) 684 WP_170946311.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52420.66 Da        Isoelectric Point: 4.8579

>NTDB_id=214897 BUW96_RS26455 WP_076816968.1 5744751..5746196(+) (rpoN/rpoN1) [Achromobacter insolitus strain DSM 23807]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLERQDEAPSAEAPAESDRESSVQDDEP
AAERETPVDEMPGSGGVYPDEDSSRPEAARPDTLREHLLGQLGLTRAAPRDVALAGLLIDELDENGYLGSPLEEILDWLP
AELEPDLDELKAALSLLQSFDPPGIGARDMAECLLLQLRNPDLEHLPEAADAAVLACARQICAQHLALLATGNAARLCAA
VGCDEATFRSAHALIRRLEPRPGRAWTVPAADYAVPDVIVRKTRGGWQVTLNSAAVPKLQINGLYAQMLGNQKESAHAGL
QSQLQQARWMIRNVEQRFDTILRVAQAIVQHQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAIQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=214897 BUW96_RS26455 WP_076816968.1 5744751..5746196(+) (rpoN/rpoN1) [Achromobacter insolitus strain DSM 23807]
ATGCAAAATATTGTACCTATGACCCGCCCAATTTTAGAACTGCGACCTGGTCAGCATTTGACGCTGACGCCACAACTGCA
GCAATCGATCCGATTGTTGCAGCTGTCCACGCTCGACCTCGAAGCCGAGATCTCGCAAGTGCTGGCGGAGAACCCGCTGC
TGGAGCGCCAGGACGAAGCGCCGTCGGCCGAGGCGCCAGCGGAATCCGACCGCGAATCCTCCGTGCAGGACGACGAACCC
GCCGCTGAGCGCGAGACGCCAGTGGATGAGATGCCGGGTTCCGGCGGCGTCTATCCCGACGAGGACTCCAGCCGGCCCGA
GGCCGCGCGCCCTGACACGCTGCGCGAGCATCTGCTGGGCCAACTGGGGCTGACGCGGGCCGCCCCGAGGGACGTCGCGC
TGGCCGGCCTCCTGATCGACGAACTGGACGAGAACGGGTACCTGGGATCGCCGCTGGAGGAAATCCTGGACTGGTTGCCG
GCGGAACTGGAGCCGGACCTGGATGAACTGAAGGCGGCCCTGTCGCTGCTCCAGTCCTTCGATCCCCCAGGCATCGGCGC
GCGCGACATGGCGGAATGCCTGCTGTTGCAGCTGCGCAACCCGGACCTGGAACACCTGCCTGAAGCGGCGGATGCCGCGG
TATTGGCCTGTGCGCGCCAGATCTGCGCGCAGCACCTGGCCCTGTTGGCGACGGGCAACGCCGCGCGCCTGTGCGCCGCC
GTGGGCTGCGATGAAGCCACGTTCCGTTCGGCCCATGCGCTGATCCGGCGCCTGGAGCCGCGTCCGGGGCGCGCCTGGAC
CGTGCCCGCGGCCGACTACGCCGTGCCCGACGTCATCGTGCGCAAGACCCGCGGCGGCTGGCAGGTGACCCTGAACAGCG
CCGCCGTGCCCAAGCTGCAGATTAACGGGCTCTACGCCCAGATGCTGGGGAACCAAAAGGAATCCGCGCACGCGGGGCTG
CAATCGCAGCTGCAGCAGGCACGCTGGATGATCCGCAATGTGGAGCAGCGCTTCGACACCATCCTGCGCGTAGCCCAGGC
CATCGTGCAGCACCAGACGGCTTTTTTCAGCCAGGGGCCTGCCGCCATGCGGCCGCTCATCCTGAAGGACATCGCTGGCG
AGCTCGGTTTGCACGAATCCACGATTTCACGGGCCACAACACAGAAGTACATGCTCACTCCGTTTGGCACGTTGGAGCTC
AAGCGCTTCTTTGGCACGGGCGTGTCGACGGACGGCGGGGATGCCACCTCGGCCACGGCGATACAGACCTTCATCCGGCA
GATGGTGGCGGAGGAGAACCGGGCCAAGCCTTTGTCCGATAGCCAGATCATGCAAAAGCTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATAGCCCCGGCGGCATTGCGCAAGGCCCAGGCGGCCGCC
CGTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.111

100

0.503

Multiple sequence alignment