Detailed information NTG0021472

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: ECs_4233
• Genome accession: NC_002695
• Coordinates: 4231789..4232997 (-)
• Length: 402 a.a.
• NCBI ID: NP_312260.2
• Uniprot ID: -
• Organism: Escherichia coli O157:H7 str. Sakai
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4226789..4237997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECs_4229	dam	4227401..4228237 (-)	837	NP_312256.1	DNA adenine methyltransferase	-
ECs_4230	damX	4228344..4229630 (-)	1287	NP_312257.1	cell division protein DamX	-
ECs_4231	aroB	4229722..4230810 (-)	1089	NP_312258.1	3-dehydroquinate synthase	-
ECs_4232	aroK	4230867..4231544 (-)	678	NP_312259.3	shikimate kinase I	-
ECs_4233	pilQ	4231789..4232997 (-)	1209	NP_312260.2	DNA catabolic fimbrial transporter	Machinery gene
ECs_4234	hofP	4232939..4233343 (-)	405	NP_312261.2	DNA catabolic protein	-
ECs_4235	hofO	4233333..4233773 (-)	441	NP_312262.1	DNA catabolic protein	-
ECs_4236	hofN	4233757..4234296 (-)	540	NP_312263.1	DNA catabolic fimbrial assembly protein	-
ECs_4237	hofM	4234280..4235074 (-)	795	NP_312264.1	DNA catabolic pilus assembly protein	-
ECs_4238	mrcA	4235194..4237746 (+)	2553	NP_312265.2	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 402 a.a.
• Molecular weight: 43575.74 Da
• Isoelectric Point: 5.8997

>NTDB_id=21472 ECs_4233 NP_312260.2 4231789..4232997(-) (pilQ) [Escherichia coli O157:H7 str. Sakai]
MLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILS
VHSIAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDNKTALSALEQW
VAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRINGRLLDLELSA
LEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQV
LQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVS
SE

Nucleotide

• Length: 1209 bp

>NTDB_id=21472 ECs_4233 NP_312260.2 4231789..4232997(-) (pilQ) [Escherichia coli O157:H7 str. Sakai]
ATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGATGGTGGATGACGTTCCGGTAGCTCAGGTGTT
GCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACGTCAGCGGTACGGTGTCGTTACATCTAACAG
ATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTGATAACGCGCCAGGAGGGCAACATTCTCTCA
GTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCT
GGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCTGGGGAGAAGCTACTGAGTGCCAAAG
GGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAACAAAACGGCGTTAAGCGCCCTTGAACAGTGG
GTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATATTGTCACCATTAATGAAAAAAGTTTGCGTGA
GTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTGGGCAAGTCACCACGCTTGGTAGCGACCTCT
CCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAACGGACGTTTGCTGGATCTTGAGCTTTCCGCG
CTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGCCTCACATCTTCAGCCTGCCAGCATTAAACA
GGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGTCGGTGGAATTTAAAGAGGCCGTCCTGGGGA
TGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTACACATCAGCCAGAACGTTCCGGGGCAGGTG
CTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGAAACGCAGGTCGAGGTCAAAAGCGGAGAAAC
GTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATAGCGTGCCGTTGCTTGGCGACATTCCCTGGT
TCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTAGTGGTGTTTATCACGCCACGACTGGTTTCC
AGTGAGTAA

Domains

Predicted by InterProScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (110-169)

• Bacterial type II and III secretion system protein (Secretin) (240-399)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Vibrio campbellii strain DS40M4	38.242	100	0.4
comE	Haemophilus influenzae Rd KW20	37.678	100	0.396
comE	Haemophilus influenzae 86-028NP	37.441	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.388
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.388
comE	Glaesserella parasuis strain SC1401	36.7	100	0.371
pilQ	Pseudomonas aeruginosa PAK	34.174	100	0.371