Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   CU076_RS20260 Genome accession   NZ_CP024830
Coordinates   3878687..3880114 (+) Length   475 a.a.
NCBI ID   WP_000980568.1    Uniprot ID   B7L9N7
Organism   Escherichia coli strain CREC-532     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3873687..3885114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU076_RS20225 (CU076_20230) yeeW 3873871..3874065 (+) 195 WP_000988600.1 DUF5983 family protein -
  CU076_RS27525 - 3874111..3874191 (+) 81 Protein_3716 hypothetical protein -
  CU076_RS20230 (CU076_20235) - 3874480..3874626 (-) 147 Protein_3717 transposase domain-containing protein -
  CU076_RS20235 (CU076_20240) - 3874680..3874862 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  CU076_RS20240 (CU076_20245) tmaR 3874963..3875292 (-) 330 WP_000450409.1 DUF496 family protein -
  CU076_RS20245 (CU076_20250) yeeA 3875464..3876522 (-) 1059 WP_001200905.1 FUSC family protein -
  CU076_RS20250 (CU076_20255) sbmC 3876720..3877193 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  CU076_RS20255 (CU076_20260) dacD 3877312..3878478 (-) 1167 WP_000830163.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  CU076_RS20260 (CU076_20265) sbcB 3878687..3880114 (+) 1428 WP_000980568.1 exodeoxyribonuclease I Machinery gene
  CU076_RS20265 (CU076_20270) tsuB 3880157..3880384 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  CU076_RS20270 (CU076_20275) tsuA 3880398..3881456 (-) 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
  CU076_RS20275 (CU076_20280) plaP 3881635..3882993 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  CU076_RS20280 (CU076_20285) yoeI 3882983..3883045 (-) 63 WP_010723108.1 membrane protein YoeI -
  CU076_RS20285 (CU076_20290) yeeY 3883260..3884189 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  CU076_RS20290 (CU076_20295) yeeZ 3884235..3885059 (-) 825 WP_000754758.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54485.76 Da        Isoelectric Point: 5.4700

>NTDB_id=214655 CU076_RS20260 WP_000980568.1 3878687..3880114(+) (sbcB) [Escherichia coli strain CREC-532]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=214655 CU076_RS20260 WP_000980568.1 3878687..3880114(+) (sbcB) [Escherichia coli strain CREC-532]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGTCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCACATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L9N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583