Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RS694_RS02215 Genome accession   NZ_CP019239
Coordinates   453896..455497 (+) Length   533 a.a.
NCBI ID   WP_029709278.1    Uniprot ID   -
Organism   Rhodoferax saidenbachensis strain DSM 22694     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448896..460497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS694_RS02190 (RS694_02190) - 449911..450564 (+) 654 WP_029709283.1 SPOR domain-containing protein -
  RS694_RS02195 (RS694_02195) - 450640..451293 (+) 654 WP_029709282.1 thiol:disulfide interchange protein DsbA/DsbL -
  RS694_RS02200 (RS694_02200) - 451402..452334 (+) 933 WP_029709281.1 exopolyphosphatase -
  RS694_RS02205 (RS694_02205) lptA 452482..453105 (+) 624 WP_029709280.1 lipopolysaccharide transport periplasmic protein LptA -
  RS694_RS02210 (RS694_02210) lptB 453102..453896 (+) 795 WP_037248208.1 LPS export ABC transporter ATP-binding protein -
  RS694_RS02215 (RS694_02215) rpoN/rpoN1 453896..455497 (+) 1602 WP_029709278.1 RNA polymerase factor sigma-54 Machinery gene
  RS694_RS02220 (RS694_02220) - 455494..456753 (+) 1260 WP_029709277.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  RS694_RS02225 (RS694_02225) - 456750..457151 (+) 402 WP_029709276.1 putative toxin-antitoxin system toxin component, PIN family -
  RS694_RS02230 (RS694_02230) - 457302..458885 (-) 1584 WP_051392078.1 methyl-accepting chemotaxis protein -
  RS694_RS02235 (RS694_02235) - 458989..460476 (-) 1488 WP_076069272.1 class I adenylate-forming enzyme family protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58946.54 Da        Isoelectric Point: 5.0254

>NTDB_id=214334 RS694_RS02215 WP_029709278.1 453896..455497(+) (rpoN/rpoN1) [Rhodoferax saidenbachensis strain DSM 22694]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLELSEERAEREEFGLGQADTPVSEGDREMEFAPN
SIADYATSTGSETKNDDEATPLSADDGPNWEGDGTTETAHDDGEWGVDAKARTNNLGDGEDKDATELARTQESLQAHLHR
QALALRLGTADSAALRFLIESLNDDGYLEDPLEDLARGLAGTEDEEQVEDLVHHFTVALGLLHSLEPVGVGARNLGECLR
LQLQALLTNASLDAPRRELLQVSLRICGQPMDLLARRDIKRLVQLCGSSDALTREAIALIARLEPKPGRRFVDVERNIVV
PDVLVMQVGKTNGGMTPKFRVQLNPDVMPRLKVHDIYANVLRNHRGGKDSASHAALQQRLQEARWFIKNIQQRFDTILRV
STAIVERQKSFFTHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTFELKYFFGSGLGTESGGNASSTAVRAL
IKQFIASESTKKPLSDNQISEMLKEQGIECARRTVAKYREGLKIAPASLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=214334 RS694_RS02215 WP_029709278.1 453896..455497(+) (rpoN/rpoN1) [Rhodoferax saidenbachensis strain DSM 22694]
ATGAAACAGGGGCTGTCCCTACGCGTCTCGCAACACCTGGCGCTCACTCCTCAACTCCAGCAATCCATCCGGTTGCTGCA
GTTGTCCACGCTCGAATTGAGCCAGGAAGTGGAGCAGATGCTCGACGAAAACCCGTTTCTCGAACTTTCGGAAGAGCGCG
CGGAACGTGAGGAGTTTGGCCTCGGTCAGGCCGACACGCCGGTCAGTGAAGGCGATCGGGAGATGGAATTTGCTCCTAAT
TCAATAGCTGATTACGCAACATCCACCGGGTCTGAGACCAAAAACGACGATGAAGCCACGCCCCTGTCGGCTGACGACGG
GCCGAACTGGGAAGGCGATGGTACGACCGAAACGGCGCACGACGATGGTGAATGGGGCGTGGACGCCAAGGCCCGTACCA
ACAACCTGGGCGATGGTGAAGACAAGGACGCCACCGAGCTGGCACGTACGCAGGAAAGCCTGCAGGCCCATCTGCACCGC
CAGGCGCTGGCGCTGCGCCTAGGTACGGCCGATAGTGCGGCTCTGCGTTTCCTGATCGAGTCGCTCAACGACGACGGTTA
TCTGGAAGACCCGCTGGAAGATCTGGCGCGCGGGCTGGCCGGCACGGAGGACGAAGAACAGGTCGAAGACCTGGTGCACC
ACTTCACCGTGGCCCTGGGCCTGCTGCACAGCCTGGAGCCCGTGGGCGTGGGCGCACGCAACCTTGGCGAATGCCTGCGC
TTGCAACTCCAGGCCCTGCTGACGAACGCGTCTCTGGACGCTCCACGCCGCGAGCTGCTGCAGGTGAGTTTGCGCATCTG
TGGTCAACCCATGGACCTGCTGGCGCGGCGCGACATCAAGCGGCTGGTGCAGCTGTGCGGCAGCAGCGATGCGCTGACAC
GTGAAGCGATTGCGCTGATCGCGCGGCTGGAGCCCAAGCCCGGGCGCCGTTTTGTGGATGTGGAGCGCAACATCGTGGTG
CCCGACGTGCTGGTCATGCAGGTCGGCAAAACCAACGGCGGCATGACACCCAAGTTCCGCGTGCAGCTGAACCCCGACGT
GATGCCGCGCCTCAAGGTGCATGACATTTACGCCAATGTGCTGCGCAACCACCGCGGCGGCAAGGACAGCGCCAGCCACG
CCGCCTTGCAGCAACGCTTGCAGGAGGCGCGTTGGTTTATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTC
AGCACCGCCATTGTGGAGCGGCAAAAAAGCTTTTTCACCCACGGCGAACTCGCCATGCGCCCGCTGGTGCTGCGCGAGAT
CGCGGATGAGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAATACATGGCCACACCGTTTGGCACGT
TTGAACTCAAGTATTTCTTTGGTTCCGGCCTGGGTACCGAATCCGGTGGCAATGCGTCCAGCACCGCAGTGCGTGCGCTG
ATCAAGCAGTTCATCGCGTCCGAGAGCACCAAGAAGCCCTTGAGTGACAACCAGATTTCCGAGATGCTCAAGGAGCAGGG
CATTGAATGTGCCCGCCGCACCGTGGCCAAATACCGCGAGGGGCTGAAGATCGCCCCCGCCTCTTTACGTAAGGCACTCT
GA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-357)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.032

100

0.51

Multiple sequence alignment