Detailed information NTG0021431

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: SERP_RS12230
• Genome accession: NC_002976
• Coordinates: 2557297..2558646 (+)
• Length: 449 a.a.
• NCBI ID: WP_000815654.1
• Uniprot ID: Q4JFB2
• Organism: Staphylococcus epidermidis RP62A
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	2557297..2578662	2557297..2558646	within	0

Gene organization within MGE regions

Location: 2557297..2578662

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SERP_RS12230 (SE2498)	ccrA	2557297..2558646 (+)	1350	WP_000815654.1	cassette chromosome recombinase CcrA	Machinery gene
SERP_RS12235 (SE2499)	ccrB	2558668..2560296 (+)	1629	WP_001186599.1	cassette chromosome recombinase CcrB	Machinery gene
SERP_RS12240 (SE2501)	-	2560814..2561164 (+)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
SERP_RS12245 (SE2503)	-	2561251..2561562 (+)	312	WP_000700853.1	DUF960 family protein	-
SERP_RS12250 (SE2504)	-	2561580..2562086 (+)	507	WP_001092169.1	DUF1643 domain-containing protein	-
SERP_RS12255 (SE2505)	-	2562107..2562424 (+)	318	WP_000659050.1	JAB domain-containing protein	-
SERP_RS12260 (SE2506)	-	2562543..2563628 (+)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
SERP_RS12265 (SE2507)	-	2563625..2565517 (+)	1893	WP_001557544.1	site-specific integrase	-
SERP_RS12270 (SE2508)	-	2565524..2565901 (+)	378	WP_000361059.1	DUF6262 family protein	-
SERP_RS12275 (SE2509)	-	2566052..2566834 (+)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
SERP_RS12280 (SE2510)	erm(A)	2566960..2567691 (-)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
SERP_RS12285 (SE2512)	-	2568225..2568887 (+)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
SERP_RS12290 (SE2513)	-	2569232..2569870 (-)	639	Protein_2380	sulfite exporter TauE/SafE family protein	-
SERP_RS12295 (SE2514)	cstR	2569870..2570130 (-)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
SERP_RS12300 (SE2515)	-	2570266..2571330 (+)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
SERP_RS12305 (SERP2516)	cstB	2571363..2572697 (+)	1335	Protein_2383	persulfide dioxygenase-sulfurtransferase CstB	-
SERP_RS12315 (SE2517)	-	2572811..2573941 (-)	1131	WP_010922808.1	ROK family transcriptional regulator	-
SERP_RS13920	psm-mec	2574275..2574343 (+)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
SERP_RS12320 (SE2519)	mecI	2574428..2574799 (-)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
SERP_RS12325 (SE2520)	mecR1	2574799..2576556 (-)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
SERP_RS12330 (SE2521)	mecA	2576653..2578662 (+)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52665.36 Da
• Isoelectric Point: 10.0809

>NTDB_id=21431 SERP_RS12230 WP_000815654.1 2557297..2558646(+) (ccrA) [Staphylococcus epidermidis RP62A]
MKQVIGYLRQSTMKQQSLAAQKQAIEAITEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQKQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQNTIQ
NIIQKRFTLNMLYPYIDEILITKSKTLMGIYFKNEPLNIVNQTTQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=21431 SERP_RS12230 WP_000815654.1 2557297..2558646(+) (ccrA) [Staphylococcus epidermidis RP62A]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAACCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTGCATGATGGTTATTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATTATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAAAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAAAACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATGCTGTATCCCTACATTGATGAAATTCTTATCACAAAAAGTAAAACACT
AATGGGTATCTATTTCAAAAATGAACCCTTAAACATCGTCAATCAAACAACGCAATCGTCAATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-248)

• Resolvase, N terminal domain (Resolvase) (4-147)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

3D structure

• AlphaFold DB: Q4JFB2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	95.323	100	0.953
ccrA	Staphylococcus aureus COL	75.501	100	0.755