Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CDW44_RS20540 Genome accession   NZ_CP024650
Coordinates   4046090..4046758 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain BH100 substr. MG2014     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4041090..4051758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDW44_RS20510 (CDW44_21165) rfaQ 4041268..4042326 (-) 1059 WP_000360397.1 lipopolysaccharide core heptosyltransferase RfaQ -
  CDW44_RS20515 (CDW44_21170) waaA 4042739..4044016 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CDW44_RS20520 (CDW44_21175) coaD 4044024..4044503 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CDW44_RS20525 (CDW44_21180) mutM 4044542..4045351 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CDW44_RS20530 (CDW44_21185) rpmG 4045449..4045616 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  CDW44_RS20535 (CDW44_21190) rpmB 4045637..4045873 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  CDW44_RS20540 (CDW44_21195) radC 4046090..4046758 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CDW44_RS20545 (CDW44_21200) coaBC 4046930..4048150 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CDW44_RS20550 (CDW44_21205) dut 4048128..4048586 (+) 459 WP_000976070.1 dUTP diphosphatase -
  CDW44_RS20555 (CDW44_21210) slmA 4048693..4049289 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  CDW44_RS20560 (CDW44_21215) pyrE 4049326..4049967 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CDW44_RS20565 (CDW44_21220) rph 4050033..4050749 (-) 717 WP_001247094.1 ribonuclease PH -
  CDW44_RS20575 (CDW44_21230) yicC 4050876..4051739 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=213980 CDW44_RS20540 WP_001297375.1 4046090..4046758(-) (radC) [Escherichia coli strain BH100 substr. MG2014]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=213980 CDW44_RS20540 WP_001297375.1 4046090..4046758(-) (radC) [Escherichia coli strain BH100 substr. MG2014]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GTTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGAG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496