Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BVG18_RS13565 Genome accession   NZ_CP019143
Coordinates   2756848..2757648 (-) Length   266 a.a.
NCBI ID   WP_005103248.1    Uniprot ID   N9QCA1
Organism   Acinetobacter lwoffii strain ZS207     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2751848..2762648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG18_RS13545 (BVG18_03360) - 2752893..2753591 (-) 699 WP_044109972.1 tetratricopeptide repeat protein -
  BVG18_RS13550 (BVG18_03365) hisS 2753622..2754917 (-) 1296 WP_044109970.1 histidine--tRNA ligase -
  BVG18_RS13555 (BVG18_03370) ispG 2754921..2756042 (-) 1122 WP_086044096.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BVG18_RS13560 (BVG18_03375) - 2756066..2756857 (-) 792 WP_004647463.1 DUF4115 domain-containing protein -
  BVG18_RS13565 (BVG18_03380) pilF 2756848..2757648 (-) 801 WP_005103248.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BVG18_RS13570 (BVG18_03385) rlmN 2757663..2758898 (-) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BVG18_RS13575 (BVG18_03390) ndk 2759021..2759452 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  BVG18_RS13580 (BVG18_03395) iscX 2759563..2759763 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  BVG18_RS13585 (BVG18_03400) - 2759851..2760402 (-) 552 WP_005107877.1 phosphatase PAP2 family protein -
  BVG18_RS13590 (BVG18_03405) - 2760423..2761703 (-) 1281 WP_086044097.1 glycosyltransferase family 1 protein -
  BVG18_RS13595 (BVG18_03410) - 2761976..2762386 (-) 411 WP_086044098.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=213391 BVG18_RS13565 WP_005103248.1 2756848..2757648(-) (pilF) [Acinetobacter lwoffii strain ZS207]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=213391 BVG18_RS13565 WP_005103248.1 2756848..2757648(-) (pilF) [Acinetobacter lwoffii strain ZS207]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCACGTGATTCTCAAGCAAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTACAAGCCAATCGCGATTCCTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCAGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGGCAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QCA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.549

100

0.695

Multiple sequence alignment