Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BHT10_RS12580 Genome accession   NZ_CP019042
Coordinates   2428496..2430004 (+) Length   502 a.a.
NCBI ID   WP_004553792.1    Uniprot ID   A0A0H3HTC0
Organism   Burkholderia pseudomallei strain 14M0960418     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2423496..2435004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHT10_RS12555 (BHT10_12430) kdsD 2424728..2425711 (+) 984 WP_004530936.1 arabinose 5-phosphate isomerase KdsD -
  BHT10_RS12560 (BHT10_12435) - 2425711..2426250 (+) 540 WP_004200146.1 KdsC family phosphatase -
  BHT10_RS12565 (BHT10_12440) lptC 2426256..2426870 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  BHT10_RS12570 (BHT10_12445) lptA 2426889..2427554 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  BHT10_RS12575 (BHT10_12450) lptB 2427551..2428327 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  BHT10_RS12580 (BHT10_12455) rpoN/rpoN1 2428496..2430004 (+) 1509 WP_004553792.1 RNA polymerase factor sigma-54 Machinery gene
  BHT10_RS12590 (BHT10_12465) hpf 2430154..2430513 (+) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BHT10_RS12595 (BHT10_12470) ptsN 2430746..2431255 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BHT10_RS12600 (BHT10_12475) hprK 2431384..2432352 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BHT10_RS12605 (BHT10_12480) rapZ 2432438..2433331 (+) 894 WP_004530937.1 RNase adapter RapZ -
  BHT10_RS12610 (BHT10_12485) - 2433358..2433990 (+) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54919.07 Da        Isoelectric Point: 4.8740

>NTDB_id=212841 BHT10_RS12580 WP_004553792.1 2428496..2430004(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 14M0960418]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPTASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=212841 BHT10_RS12580 WP_004553792.1 2428496..2430004(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 14M0960418]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCACCGCGTCCGACGACGATGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3HTC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.526

100

0.685

Multiple sequence alignment