Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BVL33_RS14795 Genome accession   NZ_CP019041
Coordinates   3079923..3080723 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain 65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3074923..3085723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVL33_RS14775 (BVL33_14775) - 3075982..3076680 (-) 699 WP_004908981.1 YfgM family protein -
  BVL33_RS14780 (BVL33_14780) hisS 3076712..3078004 (-) 1293 WP_075696795.1 histidine--tRNA ligase -
  BVL33_RS14785 (BVL33_14785) ispG 3078001..3079116 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BVL33_RS14790 (BVL33_14790) - 3079141..3079932 (-) 792 WP_004908989.1 helix-turn-helix domain-containing protein -
  BVL33_RS14795 (BVL33_14795) pilF 3079923..3080723 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BVL33_RS14800 (BVL33_14800) rlmN 3080740..3081975 (-) 1236 WP_004908993.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BVL33_RS14805 (BVL33_14805) ndk 3082097..3082528 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  BVL33_RS14810 (BVL33_14810) iscX 3082636..3082836 (-) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  BVL33_RS14815 (BVL33_14815) - 3082919..3083470 (-) 552 WP_004908998.1 phosphatase PAP2 family protein -
  BVL33_RS14820 (BVL33_14820) - 3083498..3084814 (-) 1317 WP_171261424.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=212821 BVL33_RS14795 WP_004908991.1 3079923..3080723(-) (pilF) [Acinetobacter junii strain 65]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=212821 BVL33_RS14795 WP_004908991.1 3079923..3080723(-) (pilF) [Acinetobacter junii strain 65]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGTTCCCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGTAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATCTCTTCAGAACCCGATAA
TGCCCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAGGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment