Detailed information NTG0021255

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: SAR_RS00295
• Genome accession: NC_002952
• Coordinates: 64935..66284 (-)
• Length: 449 a.a.
• NCBI ID: WP_000815654.1
• Uniprot ID: Q4JFB2
• Organism: Staphylococcus aureus subsp. aureus MRSA252
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	44919..66284	64935..66284	within	0

Gene organization within MGE regions

Location: 44919..66284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAR_RS00195 (SAR0039)	mecA	44919..46928 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
SAR_RS00200 (SAR0040)	mecR1	47025..48782 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
SAR_RS00205 (SAR0041)	mecI	48782..49126 (+)	345	WP_000369213.1	mecA-type methicillin resistance repressor MecI	-
SAR_RS15560	psm-mec	49238..49306 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
SAR_RS00210 (SAR0043)	-	49640..50770 (+)	1131	WP_111764890.1	ROK family transcriptional regulator	-
SAR_RS00220 (SAR0044)	cstB	50884..52218 (-)	1335	Protein_40	persulfide dioxygenase-sulfurtransferase CstB	-
SAR_RS00225 (SAR0046)	-	52251..53315 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
SAR_RS00230 (SAR0047)	cstR	53451..53711 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
SAR_RS00235 (SAR0048)	-	53711..54349 (+)	639	Protein_43	sulfite exporter TauE/SafE family protein	-
SAR_RS15995	-	54403..54462 (-)	60	Protein_44	DNA repair protein RadC	-
SAR_RS00240 (SAR0049)	-	54694..55356 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
SAR_RS16000	-	55748..55807 (+)	60	WP_010959190.1	erythromycin resistance leader peptide	-
SAR_RS00245 (SAR0050)	erm(A)	55890..56621 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
SAR_RS00250 (SAR0051)	-	56747..57529 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
SAR_RS00255 (SAR0052)	-	57680..58057 (-)	378	WP_000361059.1	DUF6262 family protein	-
SAR_RS00260 (SAR0053)	-	58064..59956 (-)	1893	WP_001557544.1	site-specific integrase	-
SAR_RS00265 (SAR0054)	-	59953..61038 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
SAR_RS00270 (SAR0055)	-	61157..61474 (-)	318	WP_000659050.1	JAB domain-containing protein	-
SAR_RS00275 (SAR0056)	-	61495..62001 (-)	507	WP_001092169.1	DUF1643 domain-containing protein	-
SAR_RS00280 (SAR0057)	-	62019..62330 (-)	312	WP_000700853.1	DUF960 family protein	-
SAR_RS00285 (SAR0058)	-	62417..62767 (-)	351	WP_000859155.1	SAUGI family uracil-DNA glycosylase inhibitor	-
SAR_RS00290 (SAR0059)	ccrB	63285..64913 (-)	1629	WP_001186599.1	cassette chromosome recombinase CcrB	Machinery gene
SAR_RS00295 (SAR0060)	ccrA	64935..66284 (-)	1350	WP_000815654.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52665.36 Da
• Isoelectric Point: 10.0809

>NTDB_id=21255 SAR_RS00295 WP_000815654.1 64935..66284(-) (ccrA) [Staphylococcus aureus subsp. aureus MRSA252]
MKQVIGYLRQSTMKQQSLAAQKQAIEAITEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQKQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQNTIQ
NIIQKRFTLNMLYPYIDEILITKSKTLMGIYFKNEPLNIVNQTTQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=21255 SAR_RS00295 WP_000815654.1 64935..66284(-) (ccrA) [Staphylococcus aureus subsp. aureus MRSA252]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAACCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTGCATGATGGTTATTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATTATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAAAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAAAACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATGCTGTATCCCTACATTGATGAAATTCTTATCACAAAAAGTAAAACACT
AATGGGTATCTATTTCAAAAATGAACCCTTAAACATCGTCAATCAAACAACGCAATCGTCAATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-248)

• Resolvase, N terminal domain (Resolvase) (4-147)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

3D structure

• AlphaFold DB: Q4JFB2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	95.323	100	0.953
ccrA	Staphylococcus aureus COL	75.501	100	0.755