Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BEI53_RS15685 Genome accession   NZ_CP018935
Coordinates   2894572..2895615 (+) Length   347 a.a.
NCBI ID   WP_001013197.1    Uniprot ID   A0AAE9TCD1
Organism   Bacillus cereus strain JEM-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2889572..2900615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI53_RS15650 - 2889826..2890314 (+) 489 WP_000764252.1 hypothetical protein -
  BEI53_RS15655 - 2890351..2890662 (-) 312 WP_001093243.1 hypothetical protein -
  BEI53_RS15660 - 2890915..2891130 (+) 216 WP_001008320.1 DUF3912 family protein -
  BEI53_RS15665 - 2891218..2891625 (+) 408 WP_075396044.1 hypothetical protein -
  BEI53_RS15670 - 2891801..2893195 (-) 1395 WP_001094329.1 L-cystine transporter -
  BEI53_RS15675 - 2893303..2893545 (-) 243 WP_000440711.1 DUF2626 domain-containing protein -
  BEI53_RS15680 - 2893671..2894366 (-) 696 WP_075396043.1 helix-turn-helix transcriptional regulator -
  BEI53_RS15685 comGA 2894572..2895615 (+) 1044 WP_001013197.1 competence protein ComGA Machinery gene
  BEI53_RS15690 comGB 2895608..2896639 (+) 1032 WP_000471910.1 comG operon protein ComGB -
  BEI53_RS15695 comGC 2896651..2896950 (+) 300 WP_001178696.1 comG operon protein ComGC -
  BEI53_RS15700 comGD 2896947..2897402 (+) 456 WP_000810395.1 comG operon protein ComGD -
  BEI53_RS15705 comGE 2897395..2897697 (+) 303 WP_000829456.1 competence type IV pilus minor pilin ComGE -
  BEI53_RS15710 comGF 2897667..2898137 (+) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  BEI53_RS15715 comGG 2898134..2898505 (+) 372 WP_001231192.1 competence type IV pilus minor pilin ComGG -
  BEI53_RS15720 - 2898681..2899331 (+) 651 WP_000183878.1 2OG-Fe(II) oxygenase -
  BEI53_RS15725 aroK 2899452..2899949 (+) 498 WP_000836640.1 shikimate kinase AroK -
  BEI53_RS15730 - 2899988..2900188 (+) 201 WP_000106081.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39397.95 Da        Isoelectric Point: 9.0635

>NTDB_id=212205 BEI53_RS15685 WP_001013197.1 2894572..2895615(+) (comGA) [Bacillus cereus strain JEM-2]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKILLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=212205 BEI53_RS15685 WP_001013197.1 2894572..2895615(+) (comGA) [Bacillus cereus strain JEM-2]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATACTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGATACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATTGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579

Multiple sequence alignment