Detailed information NTG0212194

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: BEI53_RS10910
• Genome accession: NZ_CP018935
• Coordinates: 2043412..2044761 (+)
• Length: 449 a.a.
• NCBI ID: WP_000879417.1
• Uniprot ID: A0AAE9TF02
• Organism: Bacillus cereus strain JEM-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2038412..2049761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BEI53_RS10885	-	2039507..2040142 (-)	636	WP_000926690.1	YigZ family protein	-
BEI53_RS10890	-	2040382..2041224 (+)	843	WP_000844766.1	DegV family protein	-
BEI53_RS10900	-	2041398..2041712 (+)	315	WP_000400857.1	winged helix-turn-helix transcriptional regulator	-
BEI53_RS10905	-	2041972..2043285 (+)	1314	WP_075396218.1	NlpC/P60 family protein	-
BEI53_RS10910	comFA	2043412..2044761 (+)	1350	WP_000879417.1	ATP-dependent helicase ComFA	Machinery gene
BEI53_RS10915	comFC	2044761..2045465 (+)	705	WP_075396217.1	comF operon protein ComFC	-
BEI53_RS10920	cspC	2045592..2045789 (+)	198	WP_001990088.1	cold shock protein CspC	-
BEI53_RS10925	hpf	2046113..2046655 (+)	543	WP_000671189.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
BEI53_RS10935	secA	2046931..2049438 (+)	2508	WP_000579368.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51233.89 Da
• Isoelectric Point: 9.5047

>NTDB_id=212194 BEI53_RS10910 WP_000879417.1 2043412..2044761(+) (comFA) [Bacillus cereus strain JEM-2]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=212194 BEI53_RS10910 WP_000879417.1 2043412..2044761(+) (comFA) [Bacillus cereus strain JEM-2]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGGAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACGGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTTGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.704	88.641	0.361