Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BEI49_RS20325 Genome accession   NZ_CP018933
Coordinates   3773493..3773927 (-) Length   144 a.a.
NCBI ID   WP_075396439.1    Uniprot ID   -
Organism   Bacillus cereus strain ISSFR-9F     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3768493..3778927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI49_RS20310 - 3769568..3770125 (+) 558 WP_075396440.1 PadR family transcriptional regulator -
  BEI49_RS20315 metE 3770742..3773030 (+) 2289 WP_001007635.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BEI49_RS20320 comJ 3773067..3773456 (-) 390 WP_000424033.1 competence protein ComJ -
  BEI49_RS20325 nucA/comI 3773493..3773927 (-) 435 WP_075396439.1 DNA-entry nuclease Machinery gene
  BEI49_RS20335 - 3774164..3774913 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  BEI49_RS20340 kinB 3774967..3776241 (-) 1275 WP_075396438.1 sporulation sensor histidine kinase KinB -
  BEI49_RS20345 - 3776552..3776752 (+) 201 WP_000929284.1 hypothetical protein -
  BEI49_RS20350 - 3776875..3778038 (+) 1164 WP_000434590.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16038.39 Da        Isoelectric Point: 8.4163

>NTDB_id=212146 BEI49_RS20325 WP_075396439.1 3773493..3773927(-) (nucA/comI) [Bacillus cereus strain ISSFR-9F]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGYSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=212146 BEI49_RS20325 WP_075396439.1 3773493..3773927(-) (nucA/comI) [Bacillus cereus strain ISSFR-9F]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGATATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment