Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   COG37_RS03495 Genome accession   NZ_CP024257
Coordinates   703413..704090 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli O25:H16 strain F5505-C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698413..709090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COG37_RS03470 istB 700023..700817 (-) 795 WP_000544809.1 IS21-like element helper ATPase IstB -
  COG37_RS03475 istA 700817..701989 (-) 1173 WP_000952428.1 IS21-like element ISEc62 family transposase -
  COG37_RS03480 - 702090..702473 (+) 384 Protein_643 hypothetical protein -
  COG37_RS03485 bfd 702642..702836 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  COG37_RS03490 bfr 702908..703384 (+) 477 WP_001735792.1 bacterioferritin -
  COG37_RS03495 pilD 703413..704090 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  COG37_RS03500 gspM 704090..704551 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  COG37_RS03505 gspL 704548..705711 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  COG37_RS03510 gspK 705726..706709 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  COG37_RS03515 gspJ 706702..707289 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  COG37_RS03520 gspI 707282..707659 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  COG37_RS03525 gspH 707656..708165 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  COG37_RS03530 gspG 708173..708610 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=212097 COG37_RS03495 WP_000178161.1 703413..704090(-) (pilD) [Escherichia coli O25:H16 strain F5505-C1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=212097 COG37_RS03495 WP_000178161.1 703413..704090(-) (pilD) [Escherichia coli O25:H16 strain F5505-C1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment