Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BVD86_RS12365 Genome accession   NZ_CP018909
Coordinates   2564239..2565039 (-) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain XJ88     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2559239..2570039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVD86_RS12345 (BVD86_12395) - 2560304..2561002 (-) 699 WP_017398816.1 YfgM family protein -
  BVD86_RS12350 (BVD86_12400) hisS 2561031..2562323 (-) 1293 WP_039764397.1 histidine--tRNA ligase -
  BVD86_RS12355 (BVD86_12405) ispG 2562320..2563435 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BVD86_RS12360 (BVD86_12410) - 2563457..2564248 (-) 792 WP_101664122.1 helix-turn-helix domain-containing protein -
  BVD86_RS12365 (BVD86_12415) pilF 2564239..2565039 (-) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BVD86_RS12370 (BVD86_12420) rlmN 2565054..2566289 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BVD86_RS12375 (BVD86_12425) ndk 2566413..2566844 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  BVD86_RS12380 (BVD86_12430) iscX 2566958..2567158 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  BVD86_RS12385 (BVD86_12435) - 2567258..2567809 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  BVD86_RS12390 (BVD86_12440) - 2567834..2569123 (-) 1290 WP_101664123.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=211751 BVD86_RS12365 WP_002114699.1 2564239..2565039(-) (pilF) [Acinetobacter pittii strain XJ88]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=211751 BVD86_RS12365 WP_002114699.1 2564239..2565039(-) (pilF) [Acinetobacter pittii strain XJ88]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCTGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744

Multiple sequence alignment