Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   A3411_RS21755 Genome accession   NZ_CP024228
Coordinates   4155634..4156626 (+) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O25:NM strain 2014EL-1343-2     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 4150634..4161626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3411_RS21725 ispD 4150695..4151405 (+) 711 WP_000246135.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  A3411_RS21730 ispF 4151405..4151884 (+) 480 WP_001735626.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  A3411_RS21735 truD 4151881..4152930 (+) 1050 WP_000568946.1 tRNA pseudouridine(13) synthase TruD -
  A3411_RS21740 surE 4152911..4153672 (+) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  A3411_RS21745 pcm 4153666..4154292 (+) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  A3411_RS21750 nlpD 4154477..4155571 (+) 1095 WP_224692580.1 murein hydrolase activator NlpD -
  A3411_RS21755 rpoS 4155634..4156626 (+) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  A3411_RS21760 ygbN 4156720..4158084 (-) 1365 WP_001735624.1 GntP family transporter -
  A3411_RS21765 ygbM 4158173..4158949 (-) 777 WP_001136934.1 HPr family phosphocarrier protein -
  A3411_RS21770 - 4158954..4159490 (-) 537 WP_050438173.1 aldolase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=211657 A3411_RS21755 WP_000081550.1 4155634..4156626(+) (rpoS) [Escherichia coli O25:NM strain 2014EL-1343-2]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=211657 A3411_RS21755 WP_000081550.1 4155634..4156626(+) (rpoS) [Escherichia coli O25:NM strain 2014EL-1343-2]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715

Multiple sequence alignment