Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   A3411_RS16450 Genome accession   NZ_CP024228
Coordinates   3124504..3125172 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O25:NM strain 2014EL-1343-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3119504..3130172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3411_RS16415 yicC 3119523..3120386 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  A3411_RS16425 rph 3120513..3121229 (+) 717 WP_001247089.1 ribonuclease PH -
  A3411_RS16430 pyrE 3121295..3121936 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  A3411_RS16435 slmA 3121973..3122569 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  A3411_RS16440 dut 3122676..3123134 (-) 459 WP_000976070.1 dUTP diphosphatase -
  A3411_RS16445 coaBC 3123112..3124332 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  A3411_RS16450 radC 3124504..3125172 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  A3411_RS16455 rpmB 3125389..3125625 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  A3411_RS16460 rpmG 3125646..3125813 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  A3411_RS16465 mutM 3125911..3126720 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  A3411_RS16470 coaD 3126759..3127238 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  A3411_RS16475 waaA 3127246..3128523 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  A3411_RS16480 rfaQ 3128936..3129994 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=211634 A3411_RS16450 WP_001297375.1 3124504..3125172(+) (radC) [Escherichia coli O25:NM strain 2014EL-1343-2]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=211634 A3411_RS16450 WP_001297375.1 3124504..3125172(+) (radC) [Escherichia coli O25:NM strain 2014EL-1343-2]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496