Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CJV63_RS07515 Genome accession   NZ_CP024223
Coordinates   1499154..1499813 (+) Length   219 a.a.
NCBI ID   WP_001221488.1    Uniprot ID   -
Organism   Escherichia coli O169:H41 strain 2014EL-1345-2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1494154..1504813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJV63_RS07490 plsC 1494300..1495037 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  CJV63_RS07495 parC 1495271..1497529 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CJV63_RS07505 ygiV 1498075..1498557 (-) 483 WP_001718874.1 GyrI-like domain-containing protein -
  CJV63_RS07510 ygiW 1498610..1499002 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CJV63_RS07515 ciaR 1499154..1499813 (+) 660 WP_001221488.1 quorum sensing response regulator transcription factor QseB Regulator
  CJV63_RS07520 qseC 1499810..1501159 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  CJV63_RS07525 ygiZ 1501210..1501542 (-) 333 WP_024171565.1 DUF2645 family protein -
  CJV63_RS07530 mdaB 1501900..1502481 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CJV63_RS07535 ygiN 1502512..1502826 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CJV63_RS07540 - 1502871..1503758 (-) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  CJV63_RS07545 - 1503755..1504702 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24692.61 Da        Isoelectric Point: 6.3527

>NTDB_id=211532 CJV63_RS07515 WP_001221488.1 1499154..1499813(+) (ciaR) [Escherichia coli O169:H41 strain 2014EL-1345-2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQCEPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211532 CJV63_RS07515 WP_001221488.1 1499154..1499813(+) (ciaR) [Escherichia coli O169:H41 strain 2014EL-1345-2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGTGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment