Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BUN12_RS01740 Genome accession   NZ_CP018902
Coordinates   297946..301650 (+) Length   1234 a.a.
NCBI ID   WP_041481719.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain HK1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 292946..306650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUN12_RS01725 (BUN12_0336) - 293009..293536 (-) 528 WP_013351758.1 ferritin-like domain-containing protein -
  BUN12_RS01730 (BUN12_0337) - 293605..294318 (-) 714 WP_013351759.1 DUF421 domain-containing protein -
  BUN12_RS01735 (BUN12_0338) addB 294459..297959 (+) 3501 WP_013351760.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BUN12_RS01740 (BUN12_0339) addA 297946..301650 (+) 3705 WP_041481719.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BUN12_RS01745 (BUN12_0340) sbcD 301717..302889 (+) 1173 WP_013351762.1 exonuclease subunit SbcD -
  BUN12_RS01750 (BUN12_0341) sbcC 302886..306278 (+) 3393 WP_013351763.1 exonuclease subunit SbcC -
  BUN12_RS01755 (BUN12_0342) - 306294..306593 (+) 300 WP_013351764.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141375.94 Da        Isoelectric Point: 5.1885

>NTDB_id=211504 BUN12_RS01740 WP_041481719.1 297946..301650(+) (addA) [Bacillus amyloliquefaciens strain HK1]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FYELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNILDDLQQIEELISCRHDFKALYERVPAVTFKRAKAVKGDEFDKVLLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEESGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTEDGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAAEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRIFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKPYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHSDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQDAIDIDEVIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTNVKRRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=211504 BUN12_RS01740 WP_041481719.1 297946..301650(+) (addA) [Bacillus amyloliquefaciens strain HK1]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCGGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTTTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAAACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTATGAGCTTGTGGACAGATATACGACGGACAGGCATGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCTGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTAGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGTGCTGATAATATTTTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAAGGCGCTGTATGAACGGGTTCCTGCGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGTGCTTTTGGACGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTTATTGAAACCCTCGTTCA
GCTCGTAATATCGTACGGAAAACGATTTGAAGCGGCGAAACAGGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCAAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGATCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGTCGGGAAATCTGTTTATGGTGGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGCTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAGATGGGGAAGGCGCCGGGCAGAAAATCGACCTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCGTATCCGCCAAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCTGAAGACTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGCGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGTTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CATCAGGTTACTTTGAAGCCGTTGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCTATCGTCGGTTGTGACGAAAATGAACTCGCCCTCATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGGGACAGACATGACGACCTGTATCAAAAACTGAGAATAT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAC
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGTCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGCATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGTCTCGGCAGAAGTTTTAATATGATGGATCTCAACAAACCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTTAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTCCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGTCCGGCGCTCATCCGTCACAGTGACATGGAGGCGCACA
GGGCTCCGGGGCTTTTGGCGCCGGAGGATATCGCGCACGACCCGGCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGCCCTGAGGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTCGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAGTACAGCGGACGCTCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACTGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGACGCAATTGATATTGATGAAGTGATTCAGTTTTTCGGCACTGAAATCGGAAAA
GACCTTGTTGGGGCCCTGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTATGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGAATTGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAATGTGAAAAGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

81.767

100

0.818

Multiple sequence alignment