Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KSH_RS05765 Genome accession   NZ_CP024180
Coordinates   1288009..1288821 (+) Length   270 a.a.
NCBI ID   WP_227522725.1    Uniprot ID   -
Organism   Moraxella osloensis strain KSH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1283009..1293821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSH_RS05750 (KSH_05745) - 1284023..1284661 (-) 639 WP_050325417.1 peroxiredoxin -
  KSH_RS05755 (KSH_05750) dusB 1284851..1285849 (-) 999 WP_099808108.1 tRNA dihydrouridine synthase DusB -
  KSH_RS05760 (KSH_05755) rlmN 1286463..1287662 (+) 1200 WP_099808109.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KSH_RS05765 (KSH_05760) pilF 1288009..1288821 (+) 813 WP_227522725.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KSH_RS05770 (KSH_05765) - 1288823..1289731 (+) 909 WP_099808110.1 helix-turn-helix domain-containing protein -
  KSH_RS05775 (KSH_05770) ispG 1289800..1290915 (+) 1116 WP_099808111.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KSH_RS05780 (KSH_05775) hisS 1290962..1292260 (+) 1299 WP_099808112.1 histidine--tRNA ligase -
  KSH_RS05785 (KSH_05780) - 1292326..1293165 (+) 840 WP_099808113.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 270 a.a.        Molecular weight: 29738.96 Da        Isoelectric Point: 9.6336

>NTDB_id=211435 KSH_RS05765 WP_227522725.1 1288009..1288821(+) (pilF) [Moraxella osloensis strain KSH]
MIGLSSFSLCLTGCTTTRQVTTQTVTASDPSRWEKNPEEIARIRTAIAAEYIRGGKLDDAKRQLESALKSYPKSAEANDM
MGVLLQQEGSPRNMQKAEAYFKNAIALNPDFSQANNNYGVYLAQLSRNQEAIAQFQIAGSALGYDGRASALENLGRTALK
VKNKPLAIQSFIKALDANRQSIVARTELIDILLTDGKFLEAQALYNDLVKIIGQANLDPRTLSQGIRLAKLANNPMEMQK
LAQQLLDRYPLSDEAKQIRSGLLTPTSTWK

Nucleotide


Download         Length: 813 bp        

>NTDB_id=211435 KSH_RS05765 WP_227522725.1 1288009..1288821(+) (pilF) [Moraxella osloensis strain KSH]
ATGATTGGCCTTTCAAGCTTCAGCCTTTGCCTAACCGGTTGTACCACTACCCGACAAGTCACCACGCAGACAGTTACTGC
CAGTGACCCCAGTCGTTGGGAAAAAAATCCAGAAGAAATCGCCAGAATTCGCACCGCGATTGCCGCTGAATATATTCGTG
GTGGTAAATTAGACGATGCCAAACGACAGTTAGAAAGTGCATTAAAGTCTTATCCAAAGTCAGCAGAAGCCAATGATATG
ATGGGCGTGTTATTGCAGCAAGAAGGCAGCCCGCGCAATATGCAAAAAGCCGAGGCTTATTTTAAAAACGCCATCGCACT
TAACCCTGACTTTAGTCAAGCAAACAACAATTATGGCGTCTATCTCGCCCAACTCAGTCGTAACCAAGAAGCGATTGCCC
AGTTTCAAATTGCCGGTTCTGCGTTAGGGTATGACGGTCGTGCCAGTGCGTTAGAGAATTTAGGGCGTACAGCATTAAAG
GTCAAAAACAAACCACTGGCGATACAGTCATTTATCAAAGCACTCGATGCCAATCGCCAAAGCATCGTCGCCCGTACAGA
GCTGATTGATATTTTATTGACCGATGGCAAATTTTTGGAGGCGCAGGCACTGTATAATGATTTGGTTAAAATTATTGGTC
AAGCCAACCTTGACCCGCGCACTTTATCGCAAGGGATTAGGCTTGCCAAACTTGCGAACAATCCCATGGAAATGCAAAAA
TTAGCGCAGCAGCTTTTAGACCGCTATCCACTAAGCGATGAAGCAAAGCAGATTCGTTCTGGACTTTTAACCCCAACGTC
AACTTGGAAATAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_16)

(93-137)

Tetratricopeptide repeat (TPR_16)

(48-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

43.396

98.148

0.426
  pilF Acinetobacter baylyi ADP1

42.586

97.407

0.415

Multiple sequence alignment