Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   LA20533_RS05085 Genome accession   NZ_CP018888
Coordinates   963318..965129 (-) Length   603 a.a.
NCBI ID   WP_054745719.1    Uniprot ID   -
Organism   Amylolactobacillus amylophilus DSM 20533 = JCM 1125 strain DSM 20533     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 958318..970129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA20533_RS05060 (LA20533_05060) - 959077..959724 (+) 648 WP_056947246.1 amino acid ABC transporter permease -
  LA20533_RS05065 (LA20533_05065) - 959735..960370 (+) 636 WP_054745724.1 amino acid ABC transporter ATP-binding protein -
  LA20533_RS05070 (LA20533_05070) - 960391..961197 (+) 807 WP_056947243.1 amino acid ABC transporter substrate-binding protein -
  LA20533_RS05075 (LA20533_05075) - 961289..961960 (+) 672 WP_141322598.1 metal ABC transporter ATP-binding protein -
  LA20533_RS05080 (LA20533_05080) - 961960..962751 (+) 792 WP_056947240.1 metal ABC transporter permease -
  LA20533_RS05085 (LA20533_05085) pepF 963318..965129 (-) 1812 WP_054745719.1 oligoendopeptidase F Regulator
  LA20533_RS05090 (LA20533_05090) - 965347..966456 (+) 1110 WP_054745717.1 LCP family protein -
  LA20533_RS05095 (LA20533_05095) - 966594..967001 (-) 408 WP_054745715.1 hypothetical protein -
  LA20533_RS05100 (LA20533_05100) - 967088..967648 (+) 561 WP_054745713.1 helix-turn-helix domain-containing protein -
  LA20533_RS05105 (LA20533_05105) - 967722..968393 (-) 672 WP_082611607.1 hypothetical protein -
  LA20533_RS05110 (LA20533_05110) - 968571..968945 (+) 375 WP_056947233.1 hypothetical protein -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68851.87 Da        Isoelectric Point: 4.7641

>NTDB_id=211312 LA20533_RS05085 WP_054745719.1 963318..965129(-) (pepF) [Amylolactobacillus amylophilus DSM 20533 = JCM 1125 strain DSM 20533]
MTDASHLPNRNDVPEELTWDLTKIFKDDAAWEAAFKDVQGELPKLEQLKGTLGNSSTTLLHGIKTILGVYRKLESVYVYA
SMKSDQDTNNSTYLALDSRASSLYAKVAAATSFFDPEVLAIDEKQLAHFIANEPDLAQYKHYLEQITRQRAHVLPAEQEK
LLAGAQDVFSASQDTFNVLTNSDMEYPFVENEDGEMVRLSNGLYSILLESTDRELRQGAFEAMYQTYGQFKNTLATTLSG
EIKRHNFMAQVHHYQDAKEAALSANNIPHVVYDNLIKAVNEHLPLLHRYVKLRKKILGVEKLHTWDMYAPLTGEPKISYT
YDEARVEAKKALAPLGPDYLEHVDEIFNNRYIDVVENKGKRSGAYSGGGYDTPPYELLNWQDNINNLYTLVHETGHSMHS
WYTRHNQEYVYGAYPIFVAEIASTTNENILTEYLLKTQTDPEVRAFILNYYLDGFKGTIFRQTQFAEFEQWIHEQDAAGQ
PLTSEVMSDFYGKLNQRYYGEALTKDPQIADEWARIPHFYYNYYVYQYSTGFAAATTLADGVTKGKEGAQEAYINYLKSG
SSMDPIDTMKQAGVDMTETAYLDQAFSIFEERLNELEQLIDHK

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=211312 LA20533_RS05085 WP_054745719.1 963318..965129(-) (pepF) [Amylolactobacillus amylophilus DSM 20533 = JCM 1125 strain DSM 20533]
GTGACCGATGCATCGCATTTACCAAACCGCAACGATGTCCCAGAGGAATTAACATGGGACTTAACTAAAATCTTCAAGGA
TGATGCTGCCTGGGAGGCCGCATTCAAAGATGTTCAGGGGGAATTACCCAAGTTAGAACAGCTAAAAGGAACACTAGGTA
ATTCTAGTACTACTTTATTACACGGGATTAAGACAATCCTTGGTGTCTACCGCAAGCTTGAGTCTGTCTATGTCTACGCC
TCGATGAAGAGTGACCAAGATACCAATAATTCAACTTACTTAGCACTCGACTCACGCGCCTCATCACTCTATGCCAAAGT
CGCTGCAGCAACTTCGTTCTTCGATCCTGAGGTCCTAGCAATTGACGAGAAGCAGCTCGCGCATTTCATTGCTAACGAGC
CAGACCTTGCTCAGTACAAGCACTACCTAGAACAGATTACCCGTCAAAGAGCACACGTACTGCCAGCCGAACAGGAGAAG
TTGTTGGCCGGTGCACAGGATGTTTTCTCAGCCTCACAAGACACCTTCAACGTGTTAACTAATTCCGATATGGAATACCC
GTTCGTCGAGAACGAGGATGGTGAGATGGTGCGTCTTTCAAACGGCCTCTACAGTATCTTGCTCGAGTCAACCGACCGTG
AACTACGCCAGGGTGCCTTTGAAGCAATGTACCAGACCTACGGCCAGTTCAAGAATACTCTAGCGACTACCCTTTCTGGT
GAAATTAAGCGCCACAACTTTATGGCCCAGGTCCACCACTATCAGGATGCGAAAGAGGCAGCTCTCAGTGCCAACAATAT
CCCACACGTGGTCTACGATAACCTCATCAAGGCGGTCAACGAACACCTGCCTCTCCTGCACAGATATGTCAAATTACGGA
AGAAAATCTTAGGTGTGGAGAAGCTCCATACTTGGGATATGTACGCCCCACTAACTGGTGAACCGAAGATTTCATACACA
TACGATGAAGCACGCGTCGAAGCAAAGAAGGCACTGGCACCACTTGGCCCAGACTACCTTGAACACGTCGACGAAATCTT
TAACAACCGCTACATCGACGTCGTCGAGAATAAAGGGAAACGCTCTGGTGCATACTCAGGTGGCGGTTATGATACCCCAC
CTTACGAATTATTGAACTGGCAAGATAATATCAACAACTTGTACACACTAGTCCACGAGACAGGCCACTCAATGCACAGC
TGGTACACGCGTCACAATCAGGAATACGTTTATGGTGCCTACCCAATCTTTGTAGCAGAAATTGCCTCAACTACAAACGA
GAACATCCTGACGGAGTATTTGCTGAAGACGCAAACGGATCCTGAGGTCCGGGCGTTCATCTTGAACTATTACCTGGATG
GCTTCAAGGGGACCATCTTCCGGCAAACCCAGTTCGCGGAATTCGAACAATGGATTCACGAGCAAGACGCTGCAGGCCAG
CCGCTCACTAGTGAAGTGATGTCAGACTTCTACGGTAAATTGAATCAGCGTTACTACGGCGAGGCCTTGACTAAGGATCC
TCAAATCGCCGACGAATGGGCGCGCATCCCCCACTTCTACTACAATTATTACGTTTACCAATACTCCACCGGCTTTGCTG
CAGCAACCACACTGGCTGACGGCGTCACGAAAGGCAAGGAGGGCGCACAGGAGGCTTACATTAACTACCTAAAATCTGGT
TCGAGCATGGATCCAATCGACACAATGAAACAAGCGGGTGTCGACATGACCGAAACGGCTTACTTGGATCAAGCATTTAG
TATTTTCGAGGAACGCTTGAATGAACTAGAACAATTAATCGACCATAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

53.12

98.342

0.522

Multiple sequence alignment