Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BUW91_RS19075 Genome accession   NZ_CP018874
Coordinates   3685129..3685503 (+) Length   124 a.a.
NCBI ID   WP_223269005.1    Uniprot ID   -
Organism   Priestia megaterium strain JX285     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3680129..3690503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUW91_RS19055 (BUW91_19060) - 3680619..3681290 (-) 672 WP_013058558.1 response regulator transcription factor -
  BUW91_RS19060 (BUW91_19065) - 3681616..3682434 (+) 819 WP_013058559.1 squalene/phytoene synthase family protein -
  BUW91_RS19065 (BUW91_19070) - 3682471..3684420 (-) 1950 WP_064472035.1 FUSC family protein -
  BUW91_RS19070 (BUW91_19075) - 3684576..3684836 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  BUW91_RS19075 (BUW91_19080) nucA/comI 3685129..3685503 (+) 375 WP_223269005.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BUW91_RS19080 (BUW91_19085) comJ 3685539..3685946 (+) 408 WP_064472036.1 competence protein ComJ -
  BUW91_RS19085 (BUW91_19090) helD 3685975..3688293 (-) 2319 WP_064472037.1 RNA polymerase recycling motor HelD -
  BUW91_RS30455 - 3688538..3688663 (-) 126 WP_016765178.1 hypothetical protein -
  BUW91_RS19090 (BUW91_19095) - 3688803..3689321 (-) 519 WP_223268980.1 enoyl-CoA hydratase/isomerase family protein -
  BUW91_RS19095 (BUW91_19100) - 3689561..3689929 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13661.90 Da        Isoelectric Point: 4.2601

>NTDB_id=211223 BUW91_RS19075 WP_223269005.1 3685129..3685503(+) (nucA/comI) [Priestia megaterium strain JX285]
MGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=211223 BUW91_RS19075 WP_223269005.1 3685129..3685503(+) (nucA/comI) [Priestia megaterium strain JX285]
ATGGGACATGCGGCGTTTTTAGCTCCTGAAAAAACGGAAGCTGAAGTAAACTACGATGCGACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

67.227

95.968

0.645

Multiple sequence alignment