Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CR540_RS00355 Genome accession   NZ_CP024155
Coordinates   72954..73622 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain 14EC047     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 67954..78622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR540_RS00325 (CR540_00365) yicC 67973..68836 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  CR540_RS00330 (CR540_00375) rph 68963..69679 (+) 717 WP_001247093.1 ribonuclease PH -
  CR540_RS00335 (CR540_00380) pyrE 69745..70386 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  CR540_RS00340 (CR540_00385) slmA 70423..71019 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CR540_RS00345 (CR540_00390) dut 71126..71584 (-) 459 WP_167586596.1 dUTP diphosphatase -
  CR540_RS00350 (CR540_00395) coaBC 71562..72782 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CR540_RS00355 (CR540_00400) radC 72954..73622 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  CR540_RS00360 (CR540_00405) rpmB 73839..74075 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CR540_RS00365 (CR540_00410) rpmG 74096..74263 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CR540_RS00370 (CR540_00415) mutM 74361..75170 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CR540_RS00375 (CR540_00420) coaD 75209..75688 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CR540_RS00380 (CR540_00425) waaA 75696..76973 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CR540_RS00385 (CR540_00430) rfaQ 77386..78444 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=211188 CR540_RS00355 WP_001298959.1 72954..73622(+) (radC) [Escherichia coli strain 14EC047]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=211188 CR540_RS00355 WP_001298959.1 72954..73622(+) (radC) [Escherichia coli strain 14EC047]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGACTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496