Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CR539_RS23905 Genome accession   NZ_CP024147
Coordinates   4607063..4607731 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 14EC033     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4602063..4612731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR539_RS23875 (CR539_23780) rfaQ 4602241..4603299 (-) 1059 WP_071999277.1 lipopolysaccharide core heptosyltransferase RfaQ -
  CR539_RS23880 (CR539_23785) waaA 4603712..4604989 (+) 1278 WP_032281849.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CR539_RS23885 (CR539_23790) coaD 4604997..4605476 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CR539_RS23890 (CR539_23795) mutM 4605515..4606324 (-) 810 WP_028132074.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CR539_RS23895 (CR539_23800) rpmG 4606422..4606589 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  CR539_RS23900 (CR539_23805) rpmB 4606610..4606846 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  CR539_RS23905 (CR539_23810) radC 4607063..4607731 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CR539_RS23910 (CR539_23815) coaBC 4607903..4609123 (+) 1221 WP_000050121.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CR539_RS23915 (CR539_23820) dut 4609101..4609559 (+) 459 WP_000976070.1 dUTP diphosphatase -
  CR539_RS23920 (CR539_23825) slmA 4609666..4610262 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CR539_RS23925 (CR539_23830) pyrE 4610299..4610940 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CR539_RS23930 (CR539_23835) rph 4611006..4611722 (-) 717 WP_001247089.1 ribonuclease PH -
  CR539_RS23940 (CR539_23845) yicC 4611849..4612712 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=211184 CR539_RS23905 WP_001297375.1 4607063..4607731(-) (radC) [Escherichia coli strain 14EC033]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=211184 CR539_RS23905 WP_001297375.1 4607063..4607731(-) (radC) [Escherichia coli strain 14EC033]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment