Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CR536_RS00395 Genome accession   NZ_CP024134
Coordinates   72852..73520 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain 14EC017     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 67852..78520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR536_RS00360 (CR536_00360) yicC 67871..68734 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  CR536_RS00370 (CR536_00370) rph 68861..69577 (+) 717 WP_001247097.1 ribonuclease PH -
  CR536_RS00375 (CR536_00375) pyrE 69643..70284 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CR536_RS00380 (CR536_00380) slmA 70321..70917 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CR536_RS00385 (CR536_00385) dut 71024..71482 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CR536_RS00390 (CR536_00390) coaBC 71460..72680 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CR536_RS00395 (CR536_00395) radC 72852..73520 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  CR536_RS00400 (CR536_00400) rpmB 73737..73973 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CR536_RS00405 (CR536_00405) rpmG 73994..74161 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CR536_RS00410 (CR536_00410) mutM 74259..75068 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CR536_RS00415 (CR536_00415) coaD 75107..75586 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CR536_RS00420 (CR536_00420) waaA 75594..76871 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CR536_RS00425 (CR536_00425) rfaQ 77284..78342 (+) 1059 WP_000360398.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=210862 CR536_RS00395 WP_001298959.1 72852..73520(+) (radC) [Escherichia coli strain 14EC017]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=210862 CR536_RS00395 WP_001298959.1 72852..73520(+) (radC) [Escherichia coli strain 14EC017]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment