Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CR535_RS00455 Genome accession   NZ_CP024131
Coordinates   83570..84238 (+) Length   222 a.a.
NCBI ID   WP_001298959.1    Uniprot ID   Q31UY8
Organism   Escherichia coli strain 14EC007     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 78570..89238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR535_RS00420 (CR535_00415) yicC 78589..79452 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  CR535_RS00430 (CR535_00425) rph 79579..80295 (+) 717 WP_001247097.1 ribonuclease PH -
  CR535_RS00435 (CR535_00430) pyrE 80361..81002 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CR535_RS00440 (CR535_00435) slmA 81039..81635 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CR535_RS00445 (CR535_00440) dut 81742..82200 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CR535_RS00450 (CR535_00445) coaBC 82178..83398 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CR535_RS00455 (CR535_00450) radC 83570..84238 (+) 669 WP_001298959.1 DNA repair protein RadC Machinery gene
  CR535_RS00460 (CR535_00455) rpmB 84455..84691 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CR535_RS00465 (CR535_00460) rpmG 84712..84879 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CR535_RS00470 (CR535_00465) mutM 84977..85786 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CR535_RS00475 (CR535_00470) coaD 85825..86304 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CR535_RS00480 (CR535_00475) waaA 86312..87589 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CR535_RS00485 (CR535_00480) rfaQ 88002..89060 (+) 1059 WP_000360398.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25274.35 Da        Isoelectric Point: 7.9748

>NTDB_id=210783 CR535_RS00455 WP_001298959.1 83570..84238(+) (radC) [Escherichia coli strain 14EC007]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=210783 CR535_RS00455 WP_001298959.1 83570..84238(+) (radC) [Escherichia coli strain 14EC007]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31UY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment