Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CR534_RS00620 Genome accession   NZ_CP024127
Coordinates   110494..111162 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 14EC001     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 105494..116162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR534_RS00585 (CR534_00575) yicC 105513..106376 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  CR534_RS00595 (CR534_00585) rph 106503..107219 (+) 717 WP_001247089.1 ribonuclease PH -
  CR534_RS00600 (CR534_00590) pyrE 107285..107926 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CR534_RS00605 (CR534_00595) slmA 107963..108559 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CR534_RS00610 (CR534_00600) dut 108666..109124 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CR534_RS00615 (CR534_00605) coaBC 109102..110322 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CR534_RS00620 (CR534_00610) radC 110494..111162 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CR534_RS00625 (CR534_00615) rpmB 111379..111615 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CR534_RS00630 (CR534_00620) rpmG 111636..111803 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CR534_RS00635 (CR534_00625) mutM 111901..112710 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CR534_RS00640 (CR534_00630) coaD 112749..113228 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CR534_RS00645 (CR534_00635) waaA 113236..114513 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CR534_RS00650 (CR534_00640) rfaQ 114926..115984 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=210704 CR534_RS00620 WP_001297375.1 110494..111162(+) (radC) [Escherichia coli strain 14EC001]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=210704 CR534_RS00620 WP_001297375.1 110494..111162(+) (radC) [Escherichia coli strain 14EC001]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCATCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496