Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BUE77_RS02115 Genome accession   NZ_CP018809
Coordinates   433516..434892 (+) Length   458 a.a.
NCBI ID   WP_006585162.1    Uniprot ID   A0A5N1I9V0
Organism   Lactobacillus jensenii strain SNUV360     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 428516..439892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUE77_RS08225 (BUE77_02085) - 429220..429396 (+) 177 WP_075362434.1 DUF308 domain-containing protein -
  BUE77_RS02090 (BUE77_02090) - 429407..430084 (+) 678 WP_006588253.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  BUE77_RS02095 (BUE77_02095) - 430169..431104 (+) 936 WP_006585165.1 AAA family ATPase -
  BUE77_RS02100 (BUE77_02100) pepC 431154..432503 (-) 1350 WP_006588710.1 aminopeptidase C -
  BUE77_RS02105 (BUE77_02105) - 432573..432863 (-) 291 WP_006585164.1 GNAT family N-acetyltransferase -
  BUE77_RS02110 (BUE77_02110) - 432963..433502 (+) 540 WP_006585163.1 dUTP diphosphatase -
  BUE77_RS02115 (BUE77_02115) radA 433516..434892 (+) 1377 WP_006585162.1 DNA repair protein RadA Machinery gene
  BUE77_RS02120 (BUE77_02120) gltX 434967..436466 (+) 1500 WP_006585161.1 glutamate--tRNA ligase -
  BUE77_RS02130 (BUE77_02130) cysS 437022..438443 (+) 1422 WP_006588250.1 cysteine--tRNA ligase -
  BUE77_RS02135 (BUE77_02135) - 438436..438879 (+) 444 WP_006585159.1 Mini-ribonuclease 3 -
  BUE77_RS02140 (BUE77_02140) rlmB 438866..439621 (+) 756 WP_006585158.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50092.73 Da        Isoelectric Point: 7.1298

>NTDB_id=210520 BUE77_RS02115 WP_006585162.1 433516..434892(+) (radA) [Lactobacillus jensenii strain SNUV360]
MAKAQTRYKCRSCGYISASYLGRCPNCGSWNQFEKEVQEVRKTSTKQTASRLMPTTGLHEPVTLEKVKAEKETRIPTKFS
ELNRVLGGGIVTGSLVLIGGDPGIGKSTLMLQIMSTLANEHKVLYVSGEESASQIKLRADRLGVGDSGMYLYPETNMSNI
RDQISDLKPDFVVIDSIQTMNEPSLDSMVGSAAQVREVTSELMHIAKIDQITVFIIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHAYRILHSVKNRFGAANEIGMFEMENSGLKEVTNPSSIFLDERLPNSTGSCVVVSLEGTRPILAEVQALVAPTA
FGYAKRTTSGIDYNKAALLLAVLEKRGNLMLQNQDAYLTTTGGIKLSEPAIDLAIVMAIGSSYKDMEIPSEDCFVGEVGL
TGEVRRVNKIESRIAEAEKIGFKRIFIPKFNMKASFANRKIEVVPVSTVAQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=210520 BUE77_RS02115 WP_006585162.1 433516..434892(+) (radA) [Lactobacillus jensenii strain SNUV360]
ATGGCAAAAGCACAAACAAGATATAAGTGTCGTTCATGTGGCTACATTTCAGCTTCATATCTAGGAAGATGTCCTAATTG
TGGCTCCTGGAACCAATTTGAAAAAGAAGTCCAAGAAGTGCGTAAGACTAGTACCAAGCAAACGGCTAGTCGTTTAATGC
CTACCACAGGCCTACATGAACCTGTAACTCTAGAAAAGGTAAAAGCTGAAAAGGAAACACGCATTCCAACTAAATTTAGT
GAACTAAATAGGGTTCTTGGTGGAGGAATTGTTACTGGTTCACTTGTTTTAATTGGTGGAGATCCAGGAATTGGTAAATC
AACATTAATGCTCCAAATTATGAGTACTTTGGCAAATGAGCATAAAGTATTATATGTTTCTGGTGAAGAATCGGCTAGCC
AAATCAAACTTAGGGCAGACCGTTTGGGTGTAGGAGATAGCGGCATGTATCTTTATCCTGAAACTAATATGAGTAACATT
CGTGATCAAATTAGTGATTTAAAGCCAGATTTTGTAGTAATTGATTCTATTCAGACAATGAATGAACCTAGTCTTGATTC
GATGGTTGGTTCAGCTGCGCAAGTTAGAGAAGTTACTAGTGAATTAATGCATATTGCTAAGATAGATCAAATCACAGTAT
TTATTATTGGACACGTGACTAAAGAAGGGGCAATTGCTGGGCCTAAGATTTTGGAACATATGGTTGACACTGTGCTCTAT
TTTGAAGGGGATGAGCATCATGCTTATCGAATTTTGCACTCAGTTAAAAATCGTTTTGGTGCAGCCAATGAAATTGGAAT
GTTTGAGATGGAAAATTCAGGATTAAAAGAAGTAACAAATCCATCTTCAATCTTCTTAGATGAGCGGTTACCTAATTCAA
CAGGGTCATGTGTAGTTGTATCACTTGAAGGAACTAGACCAATTTTAGCTGAAGTACAAGCTCTTGTAGCACCAACAGCC
TTTGGTTATGCTAAAAGGACGACCAGTGGAATTGATTATAATAAAGCAGCTTTGTTACTAGCAGTTTTAGAGAAAAGAGG
AAACTTGATGTTACAAAATCAAGATGCCTATTTAACTACGACTGGTGGGATAAAATTATCTGAGCCAGCGATTGATTTGG
CAATTGTCATGGCAATTGGTTCAAGTTATAAGGATATGGAAATTCCTTCTGAAGACTGTTTTGTTGGAGAAGTTGGCTTA
ACTGGTGAAGTACGACGTGTTAATAAAATTGAAAGCAGAATTGCTGAAGCTGAAAAAATTGGCTTTAAAAGAATTTTTAT
TCCTAAATTTAATATGAAGGCAAGTTTTGCAAATCGAAAAATTGAGGTCGTACCTGTTTCAACAGTAGCTCAAGCTTTAA
AGTTAGTTTTTGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5N1I9V0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

58.206

99.782

0.581
  radA Streptococcus pneumoniae Rx1

58.206

99.782

0.581
  radA Streptococcus pneumoniae D39

58.206

99.782

0.581
  radA Streptococcus pneumoniae R6

58.206

99.782

0.581
  radA Streptococcus pneumoniae TIGR4

58.206

99.782

0.581
  radA Streptococcus mitis SK321

58.206

99.782

0.581
  radA Bacillus subtilis subsp. subtilis str. 168

54.031

100

0.541

Multiple sequence alignment