Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   BTV20_RS03085 Genome accession   NZ_CP018805
Coordinates   614218..614757 (+) Length   179 a.a.
NCBI ID   WP_075294142.1    Uniprot ID   A0A9Q6Z0N3
Organism   Histophilus somni strain USDA-ARS-USMARC-63369     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 609218..619757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTV20_RS03065 (BTV20_03050) lpxM 609219..610172 (+) 954 WP_075294144.1 lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase -
  BTV20_RS03070 (BTV20_03055) apt 610243..610782 (+) 540 WP_012341218.1 adenine phosphoribosyltransferase -
  BTV20_RS03075 (BTV20_03060) dnaX 610813..612816 (+) 2004 WP_075294143.1 DNA polymerase III subunit gamma/tau -
  BTV20_RS03080 (BTV20_03065) - 613069..614187 (+) 1119 WP_012341220.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BTV20_RS03085 (BTV20_03070) pilF/pilF2 614218..614757 (+) 540 WP_075294142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BTV20_RS03090 (BTV20_03075) nrdA 614840..617110 (-) 2271 WP_075294141.1 class 1a ribonucleoside-diphosphate reductase subunit alpha -
  BTV20_RS03095 (BTV20_03080) - 617599..617856 (-) 258 WP_075294154.1 helix-turn-helix domain-containing protein -
  BTV20_RS03100 (BTV20_03085) - 617965..618180 (+) 216 WP_075294140.1 transposase -
  BTV20_RS10120 - 618180..618326 (+) 147 WP_157665520.1 hypothetical protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20724.72 Da        Isoelectric Point: 9.3139

>NTDB_id=210426 BTV20_RS03085 WP_075294142.1 614218..614757(+) (pilF/pilF2) [Histophilus somni strain USDA-ARS-USMARC-63369]
MRYRTIFTFIIFSLFLSACVSQPITVRFNKQQAAKARIELGLGYLKLRNFSQAKLNFDKALFYQPDYYLVHSAFAYLYQL
QGDISQAKSSYEKAISLDKSQGDVKNNYGAFLCSQGEFARAYELFEQALTTPNYYQQADTYANIIICAEAEHNIPRYQKF
LQLLKNIDPKRAEEIKKVS

Nucleotide


Download         Length: 540 bp        

>NTDB_id=210426 BTV20_RS03085 WP_075294142.1 614218..614757(+) (pilF/pilF2) [Histophilus somni strain USDA-ARS-USMARC-63369]
ATGCGATATCGGACTATATTTACTTTTATTATATTCTCTCTTTTTCTTTCTGCTTGCGTTTCTCAGCCTATAACTGTTCG
CTTTAATAAGCAACAAGCTGCAAAAGCACGAATTGAGTTAGGTTTAGGTTATTTAAAATTACGCAATTTTTCTCAAGCAA
AACTTAATTTTGATAAAGCTTTATTCTATCAACCTGACTATTATTTGGTACATTCGGCTTTTGCCTATTTATATCAATTG
CAAGGTGATATTTCGCAAGCGAAATCCTCTTATGAAAAAGCTATCAGTTTGGATAAGTCGCAAGGTGATGTGAAAAATAA
TTATGGTGCATTTTTATGTAGTCAGGGCGAATTTGCTCGTGCTTATGAGTTATTCGAGCAGGCACTTACTACGCCTAATT
ATTATCAGCAAGCGGATACCTATGCAAATATCATAATTTGTGCAGAGGCGGAACATAATATTCCACGTTATCAGAAATTT
TTACAGTTACTGAAGAATATTGATCCCAAAAGAGCGGAGGAAATAAAGAAAGTTTCTTAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-100)

Tetratricopeptide repeat (TPR_7)

(109-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.121

92.179

0.48
  pilF Glaesserella parasuis strain SC1401

44.068

98.883

0.436

Multiple sequence alignment