Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BUE86_RS17125 Genome accession   NZ_CP018785
Coordinates   3554185..3555618 (-) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain AA4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3549185..3560618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUE86_RS17095 elbB 3550718..3551371 (+) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -
  BUE86_RS17100 mtgA 3551368..3552093 (+) 726 WP_014171821.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BUE86_RS17105 npr 3552132..3552404 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  BUE86_RS17110 rapZ 3552401..3553255 (-) 855 WP_014171820.1 RNase adapter RapZ -
  BUE86_RS17115 ptsN 3553301..3553792 (-) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BUE86_RS17120 hpf 3553875..3554162 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  BUE86_RS17125 rpoN/rpoN1 3554185..3555618 (-) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  BUE86_RS17130 lptB 3555666..3556391 (-) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  BUE86_RS17135 lptA 3556398..3556952 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  BUE86_RS17140 lptC 3556921..3557496 (-) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  BUE86_RS17145 kdsC 3557493..3558059 (-) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BUE86_RS17150 kdsD 3558074..3559060 (-) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  BUE86_RS17155 - 3559074..3560051 (-) 978 WP_020883528.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=210170 BUE86_RS17125 WP_014171817.1 3554185..3555618(-) (rpoN/rpoN1) [Enterobacter ludwigii strain AA4]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=210170 BUE86_RS17125 WP_014171817.1 3554185..3555618(-) (rpoN/rpoN1) [Enterobacter ludwigii strain AA4]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCTTTCT
CCGATACCGACCGCGCGATTGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATTCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment