Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CRT55_RS04790 Genome accession   NZ_CP024056
Coordinates   730354..731013 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain SMN197SH3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 725354..736013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRT55_RS04765 (CRT55_04740) yqiA 725422..726003 (+) 582 WP_000105733.1 esterase YqiA -
  CRT55_RS04770 (CRT55_04745) parE 726032..727924 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CRT55_RS04775 (CRT55_04750) ygiN 727972..728286 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CRT55_RS04780 (CRT55_04755) mdaB 728317..728898 (-) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  CRT55_RS04785 (CRT55_04760) qseC 729008..730357 (-) 1350 WP_000673376.1 quorum sensing histidine kinase QseC -
  CRT55_RS04790 (CRT55_04765) ciaR 730354..731013 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CRT55_RS04795 (CRT55_04770) ygiW 731165..731557 (+) 393 WP_001364958.1 OB fold stress tolerance protein YgiW -
  CRT55_RS04800 (CRT55_04775) ygiV 731610..732092 (+) 483 WP_000183486.1 GyrI-like domain-containing protein -
  CRT55_RS04815 (CRT55_04785) parC 732638..734896 (+) 2259 WP_062881426.1 DNA topoisomerase IV subunit A -
  CRT55_RS04820 (CRT55_04790) plsC 735129..735866 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=209896 CRT55_RS04790 WP_001221502.1 730354..731013(-) (ciaR) [Escherichia coli strain SMN197SH3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=209896 CRT55_RS04790 WP_001221502.1 730354..731013(-) (ciaR) [Escherichia coli strain SMN197SH3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACTGGCCTTAGTAAAATGGGTTTTAGTGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TTGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment