Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   BSU_05010 Genome accession   NC_000964
Coordinates   547306..548481 (+) Length   391 a.a.
NCBI ID   NP_388382.1    Uniprot ID   P96649
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 542306..553481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_04970 (BSU04970) cwlT 544022..545011 (+) 990 NP_388378.1 ICEBs1 mobile element: two-domain autolysin with N-acetylmuramidase and DL-endopeptidase activity VirB1 -
  BSU_04980 (BSU04980) yddI 545026..545532 (+) 507 NP_388379.1 ICEBs1 mobile element: conserved protein of unknown function -
  BSU_04990 (BSU04990) yddJ 545595..545975 (+) 381 NP_388380.1 ICEBs1 mobile element: putative lipoprotein of unknown function -
  BSU_05000 (BSU05000) yddK 546166..546966 (-) 801 NP_388381.1 ICEBs1 mobile element: conserved protein of unknown function -
  BSU_05010 (BSU05010) rapF 547306..548481 (+) 1176 NP_388382.1 ICEBs1 mobile element: response regulator aspartate phosphatase Regulator
  BSU_05020 (BSU05020) phrI 548438..548557 (+) 120 NP_388383.1 ICEBs1 mobile element: secreted regulator of the activity of phosphatase RapI -
  BSU_05030 (BSU05030) yddM 548710..549651 (+) 942 NP_388384.1 ICEBs1 mobile element: putative helicase -
  BSU_05040 (BSU05040) yddN 550240..551259 (-) 1020 NP_388385.1 putative alkanal monooxygenase -
  BSU_05050 (BSU05050) lrpA 551519..551929 (+) 411 NP_388386.1 transcriptional regulator (Lrp/AsnC family) -
  BSU_05060 (BSU05060) lrpB 552052..552501 (-) 450 NP_388387.1 transcriptional regulator (Lrp/AsnC family) -
  BSU_05070 (BSU05070) yddQ 552616..553158 (+) 543 NP_388388.1 putative hydrolase -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 46566.15 Da        Isoelectric Point: 4.9688

>NTDB_id=20958 BSU_05010 NP_388382.1 547306..548481(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=20958 BSU_05010 NP_388382.1 547306..548481(+) (rapF) [Bacillus subtilis subsp. subtilis str. 168]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(10-70)

Tetratricopeptide repeat (TPR_12)

(199-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4I1A

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

38.525

93.606

0.361
  rapC Bacillus subtilis subsp. subtilis str. 168

38.108

94.629

0.361

Multiple sequence alignment