Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   BTN44_RS00350 Genome accession   NZ_CP018629
Coordinates   57246..58874 (-) Length   542 a.a.
NCBI ID   WP_001186596.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA107     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 21763..60241 57246..58874 within 0


Gene organization within MGE regions


Location: 21763..60241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTN44_RS00140 (BTN44_00145) mecR1 21966..23723 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  BTN44_RS00145 (BTN44_00150) mecA 23820..25829 (+) 2010 WP_078056052.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  BTN44_RS00150 (BTN44_00155) - 25875..26303 (-) 429 WP_000872606.1 MaoC family dehydratase -
  BTN44_RS00155 (BTN44_00160) - 26400..27143 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  BTN44_RS00165 (BTN44_00170) - 28220..28609 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  BTN44_RS00170 (BTN44_00175) - 28645..29319 (+) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  BTN44_RS00175 (BTN44_00180) - 29408..30362 (-) 955 Protein_30 replication initiation factor domain-containing protein -
  BTN44_RS00180 (BTN44_00185) mobV 30889..32130 (-) 1242 WP_000122374.1 MobV family relaxase -
  BTN44_RS00185 (BTN44_00190) tet(K) 32319..33698 (-) 1380 WP_000492283.1 tetracycline efflux MFS transporter Tet(K) -
  BTN44_RS00190 (BTN44_00195) - 33895..34569 (+) 675 WP_001105988.1 IS6-like element IS257 family transposase -
  BTN44_RS00195 (BTN44_00200) - 34688..35203 (+) 516 WP_000377078.1 hypothetical protein -
  BTN44_RS00205 (BTN44_00210) merB 35398..36048 (-) 651 WP_000790333.1 organomercurial lyase MerB -
  BTN44_RS00210 (BTN44_00215) merA 36130..37773 (-) 1644 WP_000193219.1 mercury(II) reductase -
  BTN44_RS00215 (BTN44_00220) merT 37831..38217 (-) 387 WP_001794630.1 mercuric transport protein MerT -
  BTN44_RS00220 (BTN44_00225) - 38305..38985 (-) 681 WP_000010351.1 cytochrome c biogenesis CcdA family protein -
  BTN44_RS00225 (BTN44_00230) - 38982..39335 (-) 354 WP_103067845.1 TlpA family protein disulfide reductase -
  BTN44_RS00230 (BTN44_00235) merR 39352..39759 (-) 408 WP_000425271.1 Hg(II)-responsive transcriptional regulator MerR -
  BTN44_RS00235 (BTN44_00240) - 40059..41405 (-) 1347 WP_162564149.1 dihydrolipoyl dehydrogenase family protein -
  BTN44_RS00240 (BTN44_00245) - 41688..42362 (+) 675 WP_001105999.1 IS6-like element IS257 family transposase -
  BTN44_RS00245 (BTN44_00250) - 42424..44637 (+) 2214 Protein_43 type I restriction endonuclease subunit R -
  BTN44_RS00250 (BTN44_00255) - 44717..45283 (-) 567 WP_000759939.1 PepSY domain-containing protein -
  BTN44_RS00255 (BTN44_00260) - 45433..45654 (-) 222 WP_001070883.1 hypothetical protein -
  BTN44_RS16545 - 45668..45832 (-) 165 WP_001803222.1 hypothetical protein -
  BTN44_RS00260 (BTN44_00265) - 46121..46537 (-) 417 WP_000941331.1 hypothetical protein -
  BTN44_RS00265 (BTN44_00270) - 46600..47127 (-) 528 WP_000717928.1 hypothetical protein -
  BTN44_RS00270 (BTN44_00275) - 47262..47750 (-) 489 WP_000397419.1 hypothetical protein -
  BTN44_RS00275 (BTN44_00280) - 47766..48056 (-) 291 WP_000065262.1 hypothetical protein -
  BTN44_RS00280 (BTN44_00285) - 48113..48985 (-) 873 WP_000373151.1 HTH domain-containing protein -
  BTN44_RS16670 (BTN44_00290) - 49155..49301 (-) 147 Protein_52 JAB domain-containing protein -
  BTN44_RS00290 (BTN44_00295) - 49533..50195 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  BTN44_RS16835 - 50587..50646 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  BTN44_RS00300 (BTN44_00305) erm(A) 50704..51435 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  BTN44_RS00305 (BTN44_00310) - 51561..52343 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  BTN44_RS00310 (BTN44_00315) - 52494..52871 (-) 378 WP_001041907.1 DUF6262 family protein -
  BTN44_RS00315 (BTN44_00320) - 52878..54770 (-) 1893 WP_014532403.1 site-specific integrase -
  BTN44_RS00320 (BTN44_00325) - 54965..55288 (-) 324 WP_000088091.1 JAB domain-containing protein -
  BTN44_RS00325 (BTN44_00330) - 55281..55487 (-) 207 WP_001077283.1 hypothetical protein -
  BTN44_RS00330 (BTN44_00335) - 55489..56010 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  BTN44_RS00335 (BTN44_00340) - 56029..56340 (-) 312 WP_000833212.1 DUF960 family protein -
  BTN44_RS00345 (BTN44_00345) - 56425..56775 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  BTN44_RS00350 (BTN44_00350) ccrB 57246..58874 (-) 1629 WP_001186596.1 cassette chromosome recombinase CcrB Machinery gene
  BTN44_RS00355 (BTN44_00355) ccrA 58895..60241 (-) 1347 WP_000816499.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62274.15 Da        Isoelectric Point: 9.7334

>NTDB_id=209115 BTN44_RS00350 WP_001186596.1 57246..58874(-) (ccrB) [Staphylococcus aureus strain MRSA107]
MQQLKQKRVGIYVRVSTEIQSTEGYSIDGQINQIREYCDFNNFVVVDVYADRGISGKSMNRPELQRLLKDANEGQIDSVM
VYKTNRLARNTSDLLKIVEDLHRQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSIGSVTYILSNPFYVGKIQFAK
YKDWNEKRRKGLNDKPIIAEGKHSPIIIQDLWDKVQLRKKQVSQKPQVHGKGTNLLTGIVHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVINQVLERVNQENKVDIGALNHDIAYKQQQYD
EVSGKLHNLVKTIEDNPDLTSALKATIHQYETQLNDITNQMNQLKQQQNQEKLSYDTKQIAALLQRIFQNIESMDKAQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNNTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=209115 BTN44_RS00350 WP_001186596.1 57246..58874(-) (ccrB) [Staphylococcus aureus strain MRSA107]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTTCGTGTATCAACGGAAATCCAAAGTACTGAAGGCTATAGTAT
CGATGGACAAATCAATCAAATTCGAGAATATTGTGATTTCAATAACTTTGTTGTTGTAGATGTATACGCGGATAGAGGTA
TCTCTGGAAAATCTATGAACCGACCAGAACTACAACGTTTGTTAAAAGATGCGAACGAAGGTCAGATTGATTCTGTTATG
GTCTACAAAACAAACCGACTAGCACGTAACACTTCTGACTTACTCAAAATTGTTGAAGACCTTCATCGTCAAAATGTCGA
ATTCTTCAGCTTATCTGAGCGTATGGAAGTCAATACAAGCAGTGGTAAATTGATGCTACAAATTCTAGCGAGTTTTTCAG
AATTTGAAAGAAATAATATTGTCGAAAATGTATTCATGGGTCAAACCCGACGCGCTCAAGAAGGCTATTATCAAGGCAAT
TTGCCGCTGGGCTATGACAAAATACCGGATAGCAAGCATGAACTCATGATAAACCAACATGAAGCGAATATTGTCAAATA
TATATTTGAGTCATATGCTAAAGGCCACGGATATCGTAAAATTGCGAATGCACTCAATCACAAAGGATACGTGACTAAAA
AAGGAAAGCCTTTCAGTATTGGTTCAGTGACCTATATCTTATCTAATCCATTCTATGTTGGTAAAATTCAATTCGCAAAG
TACAAAGATTGGAATGAAAAGCGTCGTAAAGGGCTGAATGATAAACCAATAATAGCTGAAGGTAAGCATTCCCCTATTAT
TATTCAAGACTTATGGGATAAAGTCCAATTACGTAAAAAACAAGTCAGTCAAAAACCTCAAGTCCACGGTAAAGGAACTA
ATCTATTAACAGGTATCGTTCATTGTCCACAATGTGGTGCACCAATGGCAGCTAGTAACACAACGAACACATTGAAAGAT
GGTACCAAGAAGCGAATACGTTATTATTCTTGCAGTAACTTCCGAAACAAAGGCTCAAAAGTATGTTCTGCGAATAGCGT
TAGAGCTGATGTGATTGAAAAATACGTCATGGATCAAATACTCGAAATTGTCAAAAGTGATAAAGTCATTAACCAAGTCT
TAGAACGTGTCAATCAAGAAAATAAAGTCGATATTGGTGCATTGAACCACGATATCGCTTATAAACAACAACAATACGAT
GAAGTCAGCGGGAAACTCCATAATTTAGTTAAAACCATTGAAGATAATCCGGACCTAACATCTGCATTGAAAGCAACTAT
TCATCAATATGAAACACAACTCAATGACATTACAAATCAAATGAATCAACTCAAACAGCAACAAAATCAAGAGAAACTAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACGAATATTTCAAAATATAGAATCAATGGATAAAGCACAACTCAAA
GCATTATATCTTACAGTCATTGACCGTATTGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTAAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATAATACCCCTCTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACGCTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

86.531

100

0.865

Multiple sequence alignment