Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BS467_RS11495 Genome accession   NZ_CP018574
Coordinates   2218486..2220819 (+) Length   777 a.a.
NCBI ID   WP_060832475.1    Uniprot ID   -
Organism   Bacillus altitudinis strain GLB197     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2213486..2225819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS467_RS11475 (BS467_11480) - 2214693..2214989 (+) 297 WP_003216788.1 post-transcriptional regulator -
  BS467_RS11480 (BS467_11485) secDF 2215031..2217259 (+) 2229 WP_017367708.1 protein translocase subunit SecDF -
  BS467_RS11485 (BS467_11490) - 2217460..2217948 (+) 489 WP_046344113.1 cation:proton antiporter regulatory subunit -
  BS467_RS11490 (BS467_11495) - 2218048..2218404 (+) 357 WP_019743029.1 LapA family protein -
  BS467_RS11495 (BS467_11500) recJ 2218486..2220819 (+) 2334 WP_060832475.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BS467_RS11500 (BS467_11505) - 2220843..2221355 (+) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  BS467_RS11505 (BS467_11510) - 2221498..2223708 (+) 2211 WP_008342726.1 RelA/SpoT family protein -
  BS467_RS11510 (BS467_11515) dtd 2223727..2224170 (+) 444 WP_008342725.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87648.39 Da        Isoelectric Point: 4.8057

>NTDB_id=208988 BS467_RS11495 WP_060832475.1 2218486..2220819(+) (recJ) [Bacillus altitudinis strain GLB197]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSQISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDTAKTMVFCFDEETKRQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=208988 BS467_RS11495 WP_060832475.1 2218486..2220819(+) (recJ) [Bacillus altitudinis strain GLB197]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGGCTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATTGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTAAAAGAACG
ATCTGCTAAGGTAGACTTTTACATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGGGCATCGCTTGTTATCACCGTAGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAACTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGGTGTGAGTATCCGTTTAAAGAGCTTGCGGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTTGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGCTT
GCAAAAAAAGGCCTGATGCAGCTTCGTCAATCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACAGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAGACAGGTGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCTCGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACATTGACAGAAGAAGATTTCATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGACTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAGCTAAAAGAAGAACTTGTCCCAGGGA
GTCAAATTTCTGTCGTTGGTGAGGTCTCTATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCC
AGAGTCGATGAATGGCAGCTCTTTGATTTACGCGGGAAAAAAGATTGGGAAAAGAAAGTATCAACACTTGATACAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAGGCAATCGGAGCTGGTGGATATTCCGATTCACCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCAGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTCTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(314-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.672

Multiple sequence alignment