Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BS467_RS03850 Genome accession   NZ_CP018574
Coordinates   771919..773298 (-) Length   459 a.a.
NCBI ID   WP_017359160.1    Uniprot ID   -
Organism   Bacillus altitudinis strain GLB197     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 766919..778298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS467_RS03830 (BS467_03845) ispF 768400..768876 (-) 477 WP_007496273.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BS467_RS03835 (BS467_03850) ispD 768880..769569 (-) 690 WP_025206612.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BS467_RS03840 (BS467_03855) - 769583..770683 (-) 1101 WP_003217214.1 PIN/TRAM domain-containing protein -
  BS467_RS03845 (BS467_03860) disA 770837..771916 (-) 1080 WP_007496267.1 DNA integrity scanning diadenylate cyclase DisA -
  BS467_RS03850 (BS467_03865) radA 771919..773298 (-) 1380 WP_017359160.1 DNA repair protein RadA Machinery gene
  BS467_RS03855 (BS467_03870) clpC 773392..775827 (-) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BS467_RS03860 (BS467_03875) - 775824..776915 (-) 1092 WP_017366451.1 protein arginine kinase -
  BS467_RS03865 (BS467_03880) - 776920..777477 (-) 558 WP_007496258.1 UvrB/UvrC motif-containing protein -
  BS467_RS03870 (BS467_03885) - 777492..777956 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49995.41 Da        Isoelectric Point: 7.1218

>NTDB_id=208964 BS467_RS03850 WP_017359160.1 771919..773298(-) (radA) [Bacillus altitudinis strain GLB197]
MAKSKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDQNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=208964 BS467_RS03850 WP_017359160.1 771919..773298(-) (radA) [Bacillus altitudinis strain GLB197]
ATGGCTAAGTCAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCCGGCTG
CGGCACTTGGAACAGTATGACAGAAGAGGTCGTTCGTAAAGAGCCGGCAAATCGTCGAAGTGCGTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCACTCGTTCTTATTGGCGGTGATCCTGGGATTGGGAAGTCCACACT
ATTATTACAAGTTTCAGCACAGCTATCAGATCAAAATCAGAATGTGTTATACATATCTGGTGAGGAATCAATCAAACAAA
CGAAGCTTAGGGCGGACCGTCTCGGTATTGAAAGTGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTTGTGGTCGATTCAATTCAAACCGTTTATCAAAGCGATATAACGTCAGC
TCCTGGATCTGTTTCTCAGGTGAGGGAATGCACTGCCCAGCTGATGAAAATTGCCAAGACGAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACGAAGGAAGGCTCGATTGCTGGGCCGCGCCTTTTAGAGCATATGGTGGATACAGTTCTTTATTTT
GAAGGGGAGCGCCATCATACGTTTCGTATTTTACGGGCAGTGAAAAACCGTTTTGGTTCAACGAATGAATTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACAGAGGTATTAAACCCATCAGAAATCTTCTTAGAAGAGCGGTCAGCAGGTGTATCTG
GATCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTAGTGGAGATACAAGCACTTATTTCACCGACGAGCTTT
GGGAATCCACGGAGAATGGCTACTGGCCTTGATCATAACCGAGTGTCATTGCTTATGGCCGTTTTAGAAAAACGGGTAGG
GCTGCTGCTGCAAAATCAAGATGCCTATTTAAAGGTCGCAGGTGGTGTGAAACTTGATGAACCAGCGATAGACTTGGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCAGATTGCTTTATAGGAGAAGTGGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCTAAACTAGGATTTAAGAGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAACCGAGAGGGATTGAATTAGTCGGTGTAGAAAATGTAGCAGAGGCACTTCGTA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.118

99.782

0.869
  radA Streptococcus mitis NCTC 12261

61.81

98.693

0.61
  radA Streptococcus pneumoniae Rx1

61.81

98.693

0.61
  radA Streptococcus pneumoniae D39

61.81

98.693

0.61
  radA Streptococcus pneumoniae R6

61.81

98.693

0.61
  radA Streptococcus pneumoniae TIGR4

61.81

98.693

0.61
  radA Streptococcus mitis SK321

61.81

98.693

0.61

Multiple sequence alignment