Detailed information NTG0208627

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BS615_RS14595
• Genome accession: NZ_CP018421
• Coordinates: 3013690..3014808 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain XDR-BJ83 isolate male patient
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3008690..3019808

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS615_RS14585 (BS615_14600)	-	3011806..3012498 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
BS615_RS14590 (BS615_14605)	pilT	3012625..3013662 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
BS615_RS14595 (BS615_14610)	pilU	3013690..3014808 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BS615_RS14600 (BS615_14615)	fur	3014878..3015315 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
BS615_RS14605 (BS615_14620)	-	3015427..3015825 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
BS615_RS14610 (BS615_14625)	-	3015880..3016203 (-)	324	WP_000442587.1	RnfH family protein	-
BS615_RS14615 (BS615_14630)	-	3016203..3017270 (-)	1068	WP_002019572.1	hypothetical protein	-
BS615_RS14625 (BS615_14640)	-	3017423..3017878 (-)	456	WP_000782976.1	bacteriohemerythrin	-
BS615_RS14630 (BS615_14645)	-	3018131..3018979 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=208627 BS615_RS14595 WP_000347039.1 3013690..3014808(+) (pilU) [Acinetobacter baumannii strain XDR-BJ83 isolate male patient]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=208627 BS615_RS14595 WP_000347039.1 3013690..3014808(+) (pilU) [Acinetobacter baumannii strain XDR-BJ83 isolate male patient]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395