Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BOC53_RS36475 Genome accession   NZ_CP018405
Coordinates   3797780..3799288 (+) Length   502 a.a.
NCBI ID   WP_085503351.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain 2013746878     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3792780..3804288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BOC53_RS36450 (BOC53_35795) kdsD 3794012..3794995 (+) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -
  BOC53_RS36455 (BOC53_35800) - 3794995..3795534 (+) 540 WP_004200146.1 KdsC family phosphatase -
  BOC53_RS36460 (BOC53_35805) lptC 3795540..3796154 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  BOC53_RS36465 (BOC53_35810) lptA 3796173..3796838 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  BOC53_RS36470 (BOC53_35815) lptB 3796835..3797611 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  BOC53_RS36475 (BOC53_35820) rpoN/rpoN1 3797780..3799288 (+) 1509 WP_085503351.1 RNA polymerase factor sigma-54 Machinery gene
  BOC53_RS36485 (BOC53_35830) hpf 3799438..3799797 (+) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BOC53_RS36490 (BOC53_35835) ptsN 3800030..3800539 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BOC53_RS36495 (BOC53_35840) hprK 3800668..3801636 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BOC53_RS36500 (BOC53_35845) rapZ 3801722..3802615 (+) 894 WP_004530937.1 RNase adapter RapZ -
  BOC53_RS36505 (BOC53_35850) - 3802773..3803867 (-) 1095 WP_085517453.1 IS5 family transposase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54904.02 Da        Isoelectric Point: 4.8740

>NTDB_id=208485 BOC53_RS36475 WP_085503351.1 3797780..3799288(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 2013746878]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETIQRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=208485 BOC53_RS36475 WP_085503351.1 3797780..3799288(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 2013746878]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCAGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.689

Multiple sequence alignment