Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BOC52_RS18280 Genome accession   NZ_CP018403
Coordinates   341942..343450 (+) Length   502 a.a.
NCBI ID   WP_085503351.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain 2013746877     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336942..348450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BOC52_RS18255 (BOC52_17890) kdsD 338174..339157 (+) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -
  BOC52_RS18260 (BOC52_17895) - 339157..339696 (+) 540 WP_004200146.1 KdsC family phosphatase -
  BOC52_RS18265 (BOC52_17900) lptC 339702..340316 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  BOC52_RS18270 (BOC52_17905) lptA 340335..341000 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  BOC52_RS18275 (BOC52_17910) lptB 340997..341773 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  BOC52_RS18280 (BOC52_17915) rpoN/rpoN1 341942..343450 (+) 1509 WP_085503351.1 RNA polymerase factor sigma-54 Machinery gene
  BOC52_RS18290 (BOC52_17925) hpf 343600..343959 (+) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BOC52_RS18295 (BOC52_17930) ptsN 344192..344701 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BOC52_RS18300 (BOC52_17935) hprK 344830..345798 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BOC52_RS18305 (BOC52_17940) rapZ 345884..346777 (+) 894 WP_004530937.1 RNase adapter RapZ -
  BOC52_RS18310 (BOC52_17945) - 346935..348029 (-) 1095 WP_085517453.1 IS5 family transposase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54904.02 Da        Isoelectric Point: 4.8740

>NTDB_id=208461 BOC52_RS18280 WP_085503351.1 341942..343450(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 2013746877]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETIQRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=208461 BOC52_RS18280 WP_085503351.1 341942..343450(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain 2013746877]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCAGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.689

Multiple sequence alignment