Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   b0247 Genome accession   NC_000913
Coordinates   264256..264732 (-) Length   158 a.a.
NCBI ID   NP_414781.1    Uniprot ID   Q47685
Organism   Escherichia coli str. K-12 substr. MG1655     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 259256..269732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  b0242 proB 260388..261491 (+) 1104 NP_414777.1 glutamate 5-kinase -
  b0243 proA 261503..262756 (+) 1254 NP_414778.1 glutamate-5-semialdehyde dehydrogenase -
  b0245 ykfI 263328..263669 (-) 342 NP_414779.1 toxin of the YkfI-YafW toxin-antitoxin system -
  b0246 yafW 263690..264007 (-) 318 NP_414780.1 antitoxin of the YkfI-YafW toxin-antitoxin pair -
  b4504 ykfH 264026..264247 (-) 222 YP_588435.1 DUF987 domain-containing protein YkfH -
  b0247 radC 264256..264732 (-) 477 NP_414781.1 RadC-like JAB domain-containing protein YkfG Machinery gene
  b0248 yafX 264748..265206 (-) 459 NP_414782.1 protein YafX -
  b0249 ykfF 265304..265543 (-) 240 NP_414783.1 protein YkfF -
  b0250 ykfB 265620..266087 (-) 468 NP_414784.1 protein YkfB -
  b0251 yafY 266110..266553 (-) 444 NP_414785.4 inner membrane lipoprotein YafY -
  b0252 yafZ 267184..268005 (-) 822 NP_414786.4 DUF932 domain-containing protein YafZ -
  b0253 ykfA 268097..268960 (-) 864 NP_414787.4 putative GTP-binding protein YkfA -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 18098.78 Da        Isoelectric Point: 8.7508

>NTDB_id=20830 b0247 NP_414781.1 264256..264732(-) (radC) [Escherichia coli str. K-12 substr. MG1655]
MKQLSFLPGEMTPQDRRLIQRALRALDRHLHEPGVAFTSTHAVREWLRLHMAALEREEFRVLYLDNQNQLIAHETLFTGT
INRTEVHPREVVKRALHFNAAAVILAHNHPSGETTPSQADKTLTQRLVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=20830 b0247 NP_414781.1 264256..264732(-) (radC) [Escherichia coli str. K-12 substr. MG1655]
ATGAAACAGCTTTCCTTTTTACCCGGCGAGATGACGCCACAGGACCGGCGTCTCATTCAGCGGGCGCTCAGGGCTCTGGA
CCGCCACCTGCATGAGCCCGGCGTAGCCTTCACCTCTACCCACGCCGTACGTGAATGGCTGCGACTGCATATGGCCGCGC
TTGAGCGGGAAGAGTTCCGGGTGTTGTATCTGGACAACCAGAATCAGTTGATTGCCCATGAAACGCTCTTCACCGGCACG
ATTAACCGCACCGAGGTGCATCCCCGGGAGGTGGTCAAACGTGCTCTGCACTTCAACGCGGCGGCGGTGATACTCGCGCA
TAACCATCCTTCCGGCGAGACGACACCTAGCCAGGCCGACAAAACCCTCACGCAGCGACTGGTTCAGGTGCTTCAGCTGG
TGGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(38-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q47685

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

57.692

65.823

0.38

Multiple sequence alignment