Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CR208_RS22500 Genome accession   NZ_CP023541
Coordinates   4600231..4601724 (-) Length   497 a.a.
NCBI ID   WP_001040629.1    Uniprot ID   -
Organism   Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4595231..4606724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR208_RS22475 (AWA97_23685) panM 4595454..4595837 (+) 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
  CR208_RS22480 (AWA97_23690) livJ 4596036..4597139 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  CR208_RS22485 (AWA97_23695) rpoH 4597410..4598264 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  CR208_RS22490 (AWA97_23700) ftsX 4598509..4599567 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  CR208_RS22495 (AWA97_23705) ftsE 4599560..4600228 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  CR208_RS22500 (AWA97_23710) pilA 4600231..4601724 (-) 1494 WP_001040629.1 signal recognition particle-docking protein FtsY Machinery gene
  CR208_RS22505 (AWA97_23715) rsmD 4601874..4602470 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  CR208_RS22510 (AWA97_23720) yhhL 4602460..4602729 (+) 270 WP_001295207.1 DUF1145 family protein -
  CR208_RS22515 (AWA97_23725) yhhM 4602732..4603091 (-) 360 WP_000042900.1 DUF2500 domain-containing protein -
  CR208_RS22520 (AWA97_23730) yhhN 4603232..4603858 (+) 627 WP_000964718.1 lysoplasmalogenase -
  CR208_RS22525 (AWA97_23735) zntA 4603932..4606130 (+) 2199 WP_000106505.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  CR208_RS22530 (AWA97_23740) tusA 4606232..4606477 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54544.36 Da        Isoelectric Point: 4.1560

>NTDB_id=208129 CR208_RS22500 WP_001040629.1 4600231..4601724(-) (pilA) [Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETAEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=208129 CR208_RS22500 WP_001040629.1 4600231..4601724(-) (pilA) [Escherichia coli O104:H21 str. CFSAN002236 strain ATCC BAA-178]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTCGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAACCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGGCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGTAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGATGTG
GGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGT
TCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACTACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGTACCGGGCAGAATGC
GGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.901

81.489

0.39

Multiple sequence alignment