Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BS103_RS02440 Genome accession   NZ_CP018332
Coordinates   510033..510833 (+) Length   266 a.a.
NCBI ID   WP_187142553.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain A1296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505033..515833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS103_RS02415 (BS103_02400) - 505943..507241 (+) 1299 WP_085940557.1 glycosyltransferase family 4 protein -
  BS103_RS02420 (BS103_02405) - 507266..507817 (+) 552 WP_032014118.1 phosphatase PAP2 family protein -
  BS103_RS02425 (BS103_02410) iscX 507917..508117 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  BS103_RS02430 (BS103_02415) ndk 508231..508662 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  BS103_RS02435 (BS103_02420) rlmN 508785..510017 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BS103_RS02440 (BS103_02425) pilF 510033..510833 (+) 801 WP_187142553.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BS103_RS02445 (BS103_02430) - 510824..511615 (+) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  BS103_RS02450 (BS103_02435) ispG 511637..512752 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BS103_RS02455 (BS103_02440) hisS 512749..514041 (+) 1293 WP_000095253.1 histidine--tRNA ligase -
  BS103_RS02460 (BS103_02445) - 514070..514768 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30080.37 Da        Isoelectric Point: 9.0242

>NTDB_id=208106 BS103_RS02440 WP_187142553.1 510033..510833(+) (pilF) [Acinetobacter baumannii strain A1296]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSNPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=208106 BS103_RS02440 WP_187142553.1 510033..510833(+) (pilF) [Acinetobacter baumannii strain A1296]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAATCCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment