Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BSL056_RS12185 Genome accession   NZ_CP018197
Coordinates   2363070..2364140 (-) Length   356 a.a.
NCBI ID   WP_073206291.1    Uniprot ID   -
Organism   Bacillus safensis strain KCTC 12796BP     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358070..2369140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSL056_RS12135 (BSL056_12165) - 2358164..2358736 (-) 573 WP_024422971.1 signal peptidase I -
  BSL056_RS12140 (BSL056_12170) tapA 2358789..2359316 (-) 528 WP_073206271.1 amyloid fiber anchoring/assembly protein TapA -
  BSL056_RS12145 (BSL056_12175) - 2359616..2359894 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  BSL056_RS12150 (BSL056_12180) - 2359929..2360123 (-) 195 WP_024422974.1 YqzE family protein -
  BSL056_RS12155 (BSL056_12185) comGG 2360183..2360566 (-) 384 WP_073206276.1 competence type IV pilus minor pilin ComGG -
  BSL056_RS12160 (BSL056_12190) comGF 2360563..2361090 (-) 528 WP_240503390.1 competence type IV pilus minor pilin ComGF -
  BSL056_RS21220 (BSL056_12195) comGE 2361002..2361316 (-) 315 WP_073206282.1 competence type IV pilus minor pilin ComGE -
  BSL056_RS12170 (BSL056_12200) comGD 2361300..2361746 (-) 447 WP_073206286.1 competence type IV pilus minor pilin ComGD -
  BSL056_RS12175 (BSL056_12205) comGC 2361739..2362032 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BSL056_RS12180 (BSL056_12210) comGB 2362049..2363089 (-) 1041 WP_073206288.1 competence type IV pilus assembly protein ComGB -
  BSL056_RS12185 (BSL056_12215) comGA 2363070..2364140 (-) 1071 WP_073206291.1 competence type IV pilus ATPase ComGA Machinery gene
  BSL056_RS12190 (BSL056_12220) - 2364317..2365192 (-) 876 WP_056767330.1 STAS domain-containing protein -
  BSL056_RS12200 (BSL056_12230) - 2365416..2365796 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BSL056_RS12205 (BSL056_12235) - 2366009..2366251 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BSL056_RS12210 (BSL056_12240) - 2366299..2366937 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -
  BSL056_RS12215 (BSL056_12245) - 2367095..2367268 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  BSL056_RS12220 (BSL056_12250) - 2367294..2367608 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  BSL056_RS12225 (BSL056_12255) - 2367685..2368938 (-) 1254 WP_073206292.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40384.85 Da        Isoelectric Point: 9.5297

>NTDB_id=207036 BSL056_RS12185 WP_073206291.1 2363070..2364140(-) (comGA) [Bacillus safensis strain KCTC 12796BP]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLKKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=207036 BSL056_RS12185 WP_073206291.1 2363070..2364140(-) (comGA) [Bacillus safensis strain KCTC 12796BP]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGCGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCACTAGCT
GTGATGCTAAAAAAAGGTCAAGTATTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACGACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACCCTTGAAGATCCTGTCGAAACAAGGAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAACGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646

Multiple sequence alignment