Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSF20_RS17605 Genome accession   NZ_CP018152
Coordinates   3454569..3455003 (-) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3449569..3460003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS17570 (BSF20_17215) - 3449582..3449935 (+) 354 WP_003154073.1 DMT family transporter -
  BSF20_RS17575 (BSF20_17220) - 3449949..3450401 (-) 453 WP_014305039.1 OsmC family protein -
  BSF20_RS17580 (BSF20_17225) - 3450462..3451169 (-) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  BSF20_RS17585 - 3451425..3451658 (-) 234 WP_024085328.1 hypothetical protein -
  BSF20_RS17590 (BSF20_17235) - 3451838..3453166 (+) 1329 WP_032857388.1 S8 family peptidase -
  BSF20_RS17595 (BSF20_17240) - 3453359..3453721 (+) 363 WP_003154082.1 hypothetical protein -
  BSF20_RS17600 (BSF20_17245) - 3453754..3454509 (-) 756 WP_003154084.1 YoaK family protein -
  BSF20_RS17605 (BSF20_17250) nucA/comI 3454569..3455003 (-) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BSF20_RS17610 (BSF20_17255) - 3455292..3456503 (+) 1212 WP_024085325.1 cytochrome P450 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=206556 BSF20_RS17605 WP_003154085.1 3454569..3455003(-) (nucA/comI) [Bacillus amyloliquefaciens strain LM2303]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=206556 BSF20_RS17605 WP_003154085.1 3454569..3455003(-) (nucA/comI) [Bacillus amyloliquefaciens strain LM2303]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment