Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   BSF20_RS15875 Genome accession   NZ_CP018152
Coordinates   3077520..3078128 (-) Length   202 a.a.
NCBI ID   WP_003153521.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3072520..3083128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS15855 (BSF20_15540) dnaD 3072757..3073455 (+) 699 WP_003153516.1 DNA replication protein DnaD -
  BSF20_RS15860 (BSF20_15545) nth 3073474..3074133 (+) 660 WP_043867236.1 endonuclease III -
  BSF20_RS15865 (BSF20_15550) - 3074130..3074621 (+) 492 WP_014305289.1 YpoC family protein -
  BSF20_RS15870 (BSF20_15555) - 3074698..3077481 (-) 2784 WP_072588468.1 PBP1A family penicillin-binding protein -
  BSF20_RS15875 (BSF20_15560) recU 3077520..3078128 (-) 609 WP_003153521.1 Holliday junction resolvase RecU Machinery gene
  BSF20_RS15880 (BSF20_15565) - 3078170..3079132 (+) 963 WP_014305287.1 DUF2515 domain-containing protein -
  BSF20_RS15885 (BSF20_15570) sspM 3079176..3079280 (-) 105 WP_003153525.1 acid-soluble spore protein SspM -
  BSF20_RS15890 (BSF20_15575) - 3079486..3079731 (+) 246 WP_003153527.1 DUF5446 family protein -
  BSF20_RS15895 (BSF20_15580) - 3079777..3080145 (+) 369 WP_003153529.1 YppE family protein -
  BSF20_RS15900 (BSF20_15585) - 3080184..3080366 (-) 183 WP_003153532.1 YppF family protein -
  BSF20_RS15905 (BSF20_15590) - 3080553..3080987 (+) 435 WP_003153534.1 YppG family protein -
  BSF20_RS15910 (BSF20_15595) - 3081017..3081430 (-) 414 WP_003153535.1 hypothetical protein -
  BSF20_RS15915 (BSF20_15600) - 3081569..3082075 (+) 507 WP_003153536.1 PTS glucose transporter subunit IIA -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=206555 BSF20_RS15875 WP_003153521.1 3077520..3078128(-) (recU) [Bacillus amyloliquefaciens strain LM2303]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=206555 BSF20_RS15875 WP_003153521.1 3077520..3078128(-) (recU) [Bacillus amyloliquefaciens strain LM2303]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATCGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.955

98.515

0.896

Multiple sequence alignment