Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   BSF20_RS14815 Genome accession   NZ_CP018152
Coordinates   2831600..2832400 (+) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2826600..2837400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS14795 (BSF20_14500) - 2826722..2827567 (+) 846 WP_015388017.1 TlyA family RNA methyltransferase -
  BSF20_RS14800 (BSF20_14505) ahrC 2827664..2828113 (+) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  BSF20_RS14805 (BSF20_14510) recN 2828145..2829875 (+) 1731 WP_024085591.1 DNA repair protein RecN Machinery gene
  BSF20_RS14810 (BSF20_14515) spoIVB 2830039..2831319 (+) 1281 WP_007408360.1 SpoIVB peptidase -
  BSF20_RS14815 (BSF20_14520) spo0A 2831600..2832400 (+) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  BSF20_RS14820 (BSF20_14525) alr 2832546..2833727 (-) 1182 WP_015388018.1 alanine racemase -
  BSF20_RS14825 (BSF20_14530) - 2833902..2834114 (-) 213 WP_014305392.1 spore germination protein -
  BSF20_RS14830 (BSF20_14535) - 2834240..2834452 (-) 213 WP_003153183.1 spore germination protein -
  BSF20_RS14835 (BSF20_14540) - 2834578..2835696 (-) 1119 WP_072588588.1 NADH-dependent flavin oxidoreductase -
  BSF20_RS20355 (BSF20_14545) - 2835790..2835927 (-) 138 WP_162918042.1 hypothetical protein -
  BSF20_RS14840 (BSF20_14550) - 2836218..2836850 (-) 633 WP_003153189.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=206554 BSF20_RS14815 WP_003153177.1 2831600..2832400(+) (spo0A) [Bacillus amyloliquefaciens strain LM2303]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=206554 BSF20_RS14815 WP_003153177.1 2831600..2832400(+) (spo0A) [Bacillus amyloliquefaciens strain LM2303]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCGGAATTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment