Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   BSF20_RS08770 Genome accession   NZ_CP018152
Coordinates   1677811..1678956 (-) Length   381 a.a.
NCBI ID   WP_077722992.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 1672811..1683956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS08725 (BSF20_08540) speE 1673102..1673932 (+) 831 WP_003151052.1 spermidine synthase -
  BSF20_RS08730 (BSF20_08545) speB 1673992..1674864 (+) 873 WP_072588367.1 agmatinase -
  BSF20_RS08735 (BSF20_08550) - 1675034..1675252 (+) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  BSF20_RS08745 (BSF20_08560) - 1675843..1676355 (-) 513 WP_003151059.1 stage II sporulation protein M -
  BSF20_RS08750 (BSF20_08565) - 1676399..1676680 (-) 282 WP_014306021.1 hypothetical protein -
  BSF20_RS08755 (BSF20_08570) - 1677089..1677292 (-) 204 WP_003151062.1 hypothetical protein -
  BSF20_RS08760 (BSF20_08575) - 1677306..1677509 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  BSF20_RS08765 (BSF20_08580) - 1677708..1677827 (-) 120 WP_015387559.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  BSF20_RS08770 (BSF20_08585) rapF 1677811..1678956 (-) 1146 WP_077722992.1 tetratricopeptide repeat protein Regulator
  BSF20_RS08775 (BSF20_08590) - 1679248..1679676 (+) 429 WP_003151069.1 DUF1934 domain-containing protein -
  BSF20_RS08780 (BSF20_08595) argS 1679673..1681343 (+) 1671 WP_072588368.1 arginine--tRNA ligase -
  BSF20_RS08785 (BSF20_08600) narK 1681478..1682668 (+) 1191 WP_003151073.1 nitrate transporter NarK -
  BSF20_RS08790 (BSF20_08605) - 1682766..1683482 (+) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45541.70 Da        Isoelectric Point: 4.6057

>NTDB_id=206521 BSF20_RS08770 WP_077722992.1 1677811..1678956(-) (rapF) [Bacillus amyloliquefaciens strain LM2303]
MASVLSSSAIGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=206521 BSF20_RS08770 WP_077722992.1 1677811..1678956(-) (rapF) [Bacillus amyloliquefaciens strain LM2303]
GTGGCGAGTGTTTTATCGTCTTCAGCGATTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTATTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCTTT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATTATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567

Multiple sequence alignment